低品位硫化铜矿生物柱浸过程细菌种群结构及演替规律  

Bacterial Population Composition and Succession in Column Bioleaching Process of Low-grade Copper Sulphide Ore

作　　者：邹平[1,2] 周兴龙[1] 张文彬[1] 林连兵[1] 张兰兰[1] 

机构地区：[1]昆明理工大学国土资源工程学院,云南昆明650039 [2]云南冶金集团总公司技术中心,云南昆明650031

出　　处：《过程工程学报》2009年第1期88-94,共7页The Chinese Journal of Process Engineering

基　　金：云南省自然科学基金资助项目(编号:2005E0068M)

摘　　要：利用分子生物学的PCR-DGGE(变性梯度凝胶电泳)分析技术和PCR-16SrDNA序列分析技术研究了以黄铜矿为主的低品位硫化铜矿中温硫杆菌柱浸过程中细菌种群结构、演替规律及与铜浸出率之间的关系.DGGE电泳图谱共6个条带中有5个条带对应的菌株16SrDNA序列与已知菌-嗜酸氧化亚铁硫杆菌A.f的同源性均为98%以上.用9K固体培养基从柱浸浸出液中随机分离出3个纯菌株的16SrDNA序列,与已知菌A.f的同源性也均为99%,均证明该生物柱浸过程以A.f为优势菌种.细菌柱浸的菌群演替发生在A.f同菌种内的各菌株之间.柱浸前期易浸的次生硫化铜矿选择了02和05两条带所对应的A.f菌株,柱浸中后期难浸的黄铜矿则选择了01,02,03,04,06五条带所对应的A.f菌株.Based on PCR-DGGE analysis, there were 5 bands in DGGE patterns of PCR amplified 16S rDNA （V3～V6） of bacterial samples of different periods in column bioleaching process of calcopyrite corresponding to bacteria which were highly related to Acidithiobacillus ferrooxidan with 98% sequence similarity. During the bioleaching of 63 d, the bacterial population succession took place. The bacterial succession came about among bacterial strains of A.f Using a 9K solid culture medium, 3 pure strains of mesophiles were isolated, which were random-sampled from the bioleaching pulp of the ore. The sequence analysis of 16S rDNA showed that 3 pure strains were highly related to A.fwith 99% sequence similarity. These results indicated that the dominant bacteria in the bioleaching process of low-grade copper sulphide ore consisting mainly of chalcopyrite was .A.f. In the early stages of bioleaching, 02 and 05 bands corresponding to bacterial strains of A.fwere chosen in the leaching of easily dissolved secondary copper sulphide ore. In the middle and latter stages of bioleaching, 01, 02, 03, 04 and 05 bands corresponding to the bacterial strains of Af were selected in the leaching of hardly dissolved primary ore. The technical parameters of PCR-DGGE analysis were optimized.

关 键 词：黄铜矿 细菌种群结构 种群演替规律 16SrDNA 序列分析 嗜酸氧化亚铁硫杆菌 

分 类 号：TF111.311[冶金工程—冶金物理化学] TF803.21