不同时间左心室注射地尔硫对大鼠顿抑心肌功能的影响  

Effect of left ventricular injection of diltiazem at different time on rat stunned myocardium

作　　者：张燕[1] 英明中[1] 李小鹰[1] 任建平[2] 韩丽娜[1] 

机构地区：[1]解放军总医院南楼心血管一科,北京100853 [2]军事医学科学院放射与辐射医学研究所

出　　处：《中华老年心脑血管病杂志》2009年第3期206-209,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家自然科学基金(30640061)

摘　　要：目的观测不同时间左心室注射地尔硫对大鼠顿抑心肌功能的影响。方法 Wistar雄性大鼠25只,随机分为5组,假手术组(A组)、顿抑心肌组(B组)、缺血期注射地尔硫组(C组)、再灌注早期注射地尔硫组(D组),再灌注晚期注射地尔硫组(E组),每组5只,观察心率(HR)、左心室收缩末期压(LVESP)、左心室舒张末期压(LVEDP)、左心室内压最大上升速率及最大下降速率(±dp/dt_(max))的变化。结果与A组大鼠比较,除C组再灌注30、60、90、120 min,D组再灌注60、90、120 min时,其他各组在缺血及再灌注后均出现HR增快,LVESP、LVEDP、±dp/dt_(max)降低(P<0.05);C组再灌注30 min、D组再灌注90 min时HR、LVESP、LVEDP、±dp/dt_(max)已恢复至术前水平。与B组大鼠比较,C组再灌注15、30、60、90、120 min,D组再灌注90、120 min HR下降,LVESP、LVEDP、±dp/dt_(max)明显改善(P<0.05)。结论在缺血和再灌注早期左心室注射地尔硫可促进顿抑心肌功能的恢复,而再灌注晚期应用则无明显作用。Objective To observe the effects of left ventricular injection of dihiazem at different time on stunned myocardium in rats. Methods Twenty-five Wistar male rats were randomly divided into 5 groups of 5 rats each：group A was sham operated, group B was myocardial stunned, group C was injected with dihiazem into left ventricle during ischemia while group D was injected during early reperfusion and group E was injected at late stage of reperfusion. The heart rate （HR）, left ventricular end systolic pressure （LVESP）, left ventricular end diastolic pressure （LVEDP）, left ventricular pressure maximum upstroke velocity and descending velocity （±dp/dtmax） of each group were observed. Results Compared with group A, the HR of other groups increased and LVESP, LVEDP and ±dp/dtmax descended except reperfusion for 30,60,90 and 120 min in group C and reperfusion for 90 and 120 min in group D （P〈 0.05）. After reperfusion for 30 min in group C and after reperfusion for 90 min in group D,the HR,LVESP,LVEDP and ±dp/dt recovered to the preoperative baseline values. Compared with group B,after reperfusion for 15,30,60,90 and 120 rain in group C and after reperfusion for 90 and 120 min in group D,the HR descended, LVESP, LVEDP and ±dp/dt were improved. Conclusion Injection of dihiazem into left ventricle during ischemia or early reperfusion can promote the recovery of stunned myocardial function, but injection at late stage of reperfusion has no effect on cardiac function.

关 键 词：地尔硫(艹卓) 心肌顿抑 心肌再灌注 心室功能 左 

分 类 号：R965[医药卫生—药理学]