Using the nematode Caenorhabditis elegans as a model animal for assessing the toxicity induced by microcystin-LR  

作　　者：LI Yunhui WANG Yang YIN Lihong PU Yuepu WANG Dayong 

机构地区：[1]School of Public Health, Southeast University, Nanjing 210009, China [2]Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Nanjing 210009, China [3]Department of Genetics and Developmental Biology, Southeast University, Nanjing 210009, China

出　　处：《Journal of Environmental Sciences》2009年第3期395-401,共7页环境科学学报（英文版）

基　　金：supported by the National Natural Sci-ence Foundation of China (No. 30771113, 30870810);the Doctoral Program of Higher Education of China (No.20050286035);the Jiangsu 333 Project Foundation (No.07056);the Natural Science Foundation of Jiangsu Province (No. BK2006107, BK2008320)

摘　　要：Among more than 75 variants of microcystin （MC）, microcystin-LR （MC-LR） is one of the most common toxins. In this study, the feasibility of using Caenorhabditis elegans to evaluate MC-LR toxicity was studied. C. elegans was treated with MC-LR at different concentrations ranging from 0.1 to 80 μg/L. The results showed that MC-LR could reduce lifespan, delay development, lengthen generation time, decrease brood size, suppress locomotion behavior, and decreases hsp-16-2-gfp expression. The endpoints of generation time, brood size, and percentage of the population expressing hsp-16-2-gfp were very sensitive to 1.0μg/L of MC-LR, and would be more useful for the evaluation of MC-LR toxicity. Furthermore, the tissue-specific hsp-16-2-gfp expressions were investigated in MC-LR-exposed animals, and the nervous system and intestine were primarily affected by MC-LR. Therefore, the generation time, brood size, and hsp-16-2-gfp expression in C. elegans can be explored to serve as valuable endpoints for evaluating the potential toxicity from MC-LR exposure.Among more than 75 variants of microcystin （MC）, microcystin-LR （MC-LR） is one of the most common toxins. In this study, the feasibility of using Caenorhabditis elegans to evaluate MC-LR toxicity was studied. C. elegans was treated with MC-LR at different concentrations ranging from 0.1 to 80 μg/L. The results showed that MC-LR could reduce lifespan, delay development, lengthen generation time, decrease brood size, suppress locomotion behavior, and decreases hsp-16-2-gfp expression. The endpoints of generation time, brood size, and percentage of the population expressing hsp-16-2-gfp were very sensitive to 1.0μg/L of MC-LR, and would be more useful for the evaluation of MC-LR toxicity. Furthermore, the tissue-specific hsp-16-2-gfp expressions were investigated in MC-LR-exposed animals, and the nervous system and intestine were primarily affected by MC-LR. Therefore, the generation time, brood size, and hsp-16-2-gfp expression in C. elegans can be explored to serve as valuable endpoints for evaluating the potential toxicity from MC-LR exposure.

关 键 词：MICROCYSTIN-LR toxicity assessment brood size stress response Caenorhabditis elegans 

分 类 号：X52[环境科学与工程—环境工程] Q343.1[生物学—遗传学]