过氧化物酶活性部位的模拟及其催化显色反应的研究  

Studies on the Mimetic Active Centre of Peroxidase and Catalytic Color Reaction

作　　者：孙淑声[1] 王爱民[1] 

机构地区：[1]北京大学化学系

出　　处：《北京大学学报（自然科学版）》1989年第6期692-697,共6页Acta Scientiarum Naturalium Universitatis Pekinensis

基　　金：国家自然科学基金

摘　　要：本文合成了锰(Ⅲ)与meso-四(4-磺基苯)卟啉的螯合物(Mn-TPPS_4),研究了它作为过氧化物模拟酶对过氧化氢、4-氨基安替比林与苯酚显色反应的催化活性。拟订的光度法可准确地测定0～6μg/ml H_2O_2含量,1.5μg、8.0μg和40μgH_2O_2的平均回收率依次为95％、96％和100％。将显色反应与葡萄糖氧化酶催化反应偶联,测定了人血清中葡萄糖的含量,取得了满意的结果。The chelate compound of manganese tetra-(4-sulphophenyl) porphine (Mn-TPPS4) has been synthesized. The catalytic characteristics of Mn-TPPS4 as mimesis of peroxi-dase on chromogenic reaction of hydrogen peroxide with 4-aminoantipyrine and phenol have been studied. Trace of hydrogen peroxide can be determined with accuracy and precision. The glucose in human serum has been determined by the proposed reaction combining with catalytic reaction of glucose oxidase with satisfactory results.

关 键 词：模拟酶 催化显色反应 葡萄糖 

分 类 号：TQ426.97[化学工程]