菜青虫N-乙酰-β-D-氨基葡萄糖苷酶的抑制剂筛选  

Filtration of the Cabbage Butterfly(Pieris rapae) N-Acetyl-β-D-Glucosaminidase Inhibitor

作　　者：叶志云[1] 柯莉娜[1] 黄小红[2] 林祥明[1] 石艳[1] 

机构地区：[1]厦门大学生命科学学院．细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005 [2]福建农林大学动物科学学院,福建福州350002

出　　处：《厦门大学学报（自然科学版）》2009年第2期260-264,共5页Journal of Xiamen University：Natural Science

基　　金：福建省自然科学基金(2006J0078);厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室开放项目(2007102)资助

摘　　要：以菜青虫N-乙酰-β-D-氨基葡萄糖苷酶(NAGase)为研究对象,比较了不同菜青虫部位来源及不同生长期的菜青虫NAGase活性.结果表明,5龄虫和预蛹表皮来源的NAGase比活最高.选取肉桂酸等天然化合物及其衍生物以及一些具有抗虫或杀虫活性的植物的粗提物,以其对菜青虫NAGase的抑制作用为指标来粗筛NAGase抑制剂.结果表明茴香酸、异丙基苯甲酸、间-羟基苯甲酸、2,4-二甲氧基苯甲酸以及肉桂酸对该酶具有一定的抑制效果,6 mmol/L浓度可以分别使酶活力被抑制35.3%、25.9%、26.7%、29.1%和20.0%;银杏外种皮粗提物有较强的抑制效果,0.4 mg/mL的银杏酸可以让酶活力几乎完全丧失.上述结果提示有望从粗筛得到的几种植物中进一步分离纯化出高效的NAGase抑制剂,为开发新型生物农药提供理论依据.We compared the specific activity of N-Acetyl-β-D-glucosaminidase （NAGase） from the larva of cabbage butterfly （Pieris rapae） ,the results showed that the specific activity of NAGase from the epidermis of cabbage butterfly larva was the highest among the specific activities of the NAGase from cabbage butterfly larva different parts,and the specific activity of NAGase from the five-age larva and pre-pupae are higher than other stages of the cabbage butterfly. Through the research on inhibitor effects of natural compounds and their derivants on NAGase activity and crude extract from plants on NAGase activity, the result indicated p-anisic acid, p-lsopropyl benzoic acid, m- Hydroxy benzoic acid, 2,4-Dimethoxy benzoic acid and cinnamic acid, each 6 mmol/L,can obtain inhibit rate 35.3 %, 25.9 %, 26.7 %, 29.1 % and 20.00%, respectively. And the effects of other compounds are far from satisfactory. The research on the inhibition effect of plants crude extract showed that crude extracts from the episperm of gingko billoba with water had strong inhibition effects. This research would serve as a theoretic basis for the development of bio-pesticide which had the major components of insect chitinolytic enzyme inhibitors.

关 键 词：菜青虫 N-乙酰-Β-D-氨基葡萄糖苷酶 抑制剂筛选 

分 类 号：Q356.1[生物学—遗传学]