不同季节锯缘青蟹内表皮N-乙酰-β-D-氨基葡萄糖苷酶活力与性质的比较  

Investigation on the Activity and Basic Properties of NAGase from Endepidermis of the Green Crab(Scylla serrata)During Different Seasons

作　　者：颜雅雯[1] 王勤[1] 石艳[1] 王烨[1] 谢进金[2] 陈清西[1] 

机构地区：[1]厦门大学生命科学学院,细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005 [2]泉州师范学院生物学系,福建泉州362000

出　　处：《厦门大学学报（自然科学版）》2009年第2期265-268,共4页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金(40576066);福建省泉州市科技计划项目(2007N5)资助

摘　　要：研究同一生长期不同季节的锯缘青蟹内表皮N-乙酰-β-D-氨基葡萄糖苷酶（NAGase，EC3．2．1．52）的活力与部分性质．结果表明：锯缘青蟹内表皮NAGase活力变化的趋势表现出一定的季节性规律。在一年当中，酶活力较高的月份为10～12月·3月份的活力较低．然后活力逐渐升高。直至11月，活力达到最高．此后又逐渐减弱．研究该酶的基本酶学性质,在不同的季节表现都比较稳定．最适pH在5．6～5．8之间，pH稳定性维持在5．0～10．0，最适温度为45℃，温度稳定性的研究表明，在大于45℃的条件下酶活力逐渐丧失．The N-Acetyl-β-D-glucosaminidase （NAGase, EC 3.2.1.52） catalyzes the cleavage of N-acetylglucosamine polymers. It is a composition of the chitinases and cooperates with endo-chitinase and exo-chitinase to disintegrate chitinin to N-acetylglueosamine. It is widely distributed in animal tissues and in microorganisms. Green crab is a very typical crustacean for mariculture in China. However,to our best knowledge,no study about tracking detection of chitinolytic enzymes from green crab during different seasons has been reported yet. The variation of activity and basic properties of NAGase from endepidermis of the green crab during different seasons was investigated in this paper. NAGase was extracted with 0.01 mol/L Tris-HCl buffer （pH 7.5） containing 0.2 mol/L NaCl. The results showed that the activities of NAGase from endepidermis exhibited a synchronously evident rhythm. The activity showed a bell-shaped evolution and reached its top value in November. It was found that during different seasons,the optimum pH for this enzyme was 5.6-5.8, and the optimum temperature was 45℃. Besides, the enzyme was stable at pH 5.0 - 10.0, and below 45 ℃. These results would be useful for cultivation of green crab.

关 键 词：不同季节 锯缘青蟹 内表皮 NAGASE 活力 性质 

分 类 号：Q356.1[生物学—遗传学]