PCR方法检测猪园环病毒2型感染  

Detection of PCV-2 Infection in Swine by PCR

作　　者：何后军[1] 罗咏梅[1] 戴益民[1] 邓舜洲[1] 

机构地区：[1]江西农业大学动物科技学院,江西南昌330045

出　　处：《江西农业大学学报》2009年第1期5-7,共3页Acta Agriculturae Universitatis Jiangxiensis

基　　金：江西省科技厅2005年重大招标项目(赣科发计字[2005]73号);江西省教育厅项目(赣教高字[2008]3号)

摘　　要：参考GenBank已发表的PCV-2基因序列,根据PCV-2(AF027217)和PCV-1(U49186)全基因序列设计合成一对引物,进行PCR扩增,产物经琼脂糖凝胶电泳,呈现一条大小约1 154 bp的特异条带,经条件优化,建立了猪病料中PCV-2感染的PCR检测方法。采用建立的PCR方法对江西各地猪场133份猪的病料进行PCV-2检测,结果表明,受检猪的组织及血清样品中PCV-2的总检出率为53.38%,证实江西各地猪场存在PCV-2感染。To construct a specific PCR detecting method, a pair of primers were designed to amplify a partial fragment of PCV - 2, referring to PCV - 2 （ AF027217 ） and PCV - 1 （ U49186） complete genome published in Genbank. The PCR product was about 1 154bp in size detected by gelose gel electrophoresis. 133 diseased or dead swine specimens from different pig farms in Jiangxi were detected by the PCR for PCV -2. It showed 71 specimens were positive for PCV -2, the positive rate was 53.38%, it proved that there was PCV -2 infection at different pig farms in Jiangxi.

关 键 词：猪圆环病毒2型 感染 PCR 检测 

分 类 号：S858.28[农业科学—临床兽医学] S852.659.2[农业科学—兽医学]