化妆品中7种氟喹诺酮类药物的反相高效液相色谱法测定  

Determination of Seven Fluoroquinolones in Cosmetics by Reversed-phase High Performance Liquid Chromatography

作　　者：郑艳明 郭新东 冼燕萍 杜志峰 罗海英 吴玉銮 李慧勇 

机构地区：[1]广州市质量监督检测研究院,广东广州510110

出　　处：《分析测试学报》2009年第2期235-238,共4页Journal of Instrumental Analysis

基　　金：国家质检总局科技计划资助项目(2007QK302);广东省科技计划资助项目(2008B080600007)

摘　　要：建立了反相高效液相色谱同时测定膏霜类化妆品中7种氟喹诺酮类药物（诺氟沙星、培氟沙星、环丙沙星、丹诺沙星、恩诺沙星、沙拉沙星、双氟沙星）的方法。样品先用乙腈-水（0.1%甲酸）（体积比5∶95）溶液提取,经正己烷脱脂净化后用反相高效液相色谱法测定。添加水平为1.0～10.0 mg/kg时,回收率为87%～108%,相对标准偏差为2.3%～5.8%。结果表明,该法操作简便、灵敏、准确,适用于膏霜类化妆品中氟喹诺酮类药物的测定。A reversed-phase high performance liquid chromatography（RP-HPLC） method was established for the determination of seven fluoroquinolones, such as norfloxacin, pefloxacin, ciprofloxacin, danofloxacin, enrofloxacin, sarafloxacin and difloxacin, in moisturizers and creams cosmetics. Cosmetic sample was extracted with acetonitrile-0. 1% formic acid（5 ： 95, by volume）, and then the extract was quantified by reverse phase high performance liquid chromatography after it was purified by hexane. The calibration curves showed good linearity in the range of 0.5 - 5.0 mg ·L^-1 for pefloxacin, ciprofloxacin, and difloxacin, 0.2 -5.0 mg ·L^-1 for the rest of analytes. The recoveries of seven fluoroquinolones from spiked samples at three concentration levels of 1, 5, 10 mg/kg were ranged from 87% to 108% with RSDs of 2. 3% -5.8%. The results indicated that the method was simple, sensitive and accurate, and was suitable for the determination of fluoroquinolones in cosmetic sample.

关 键 词：反相高效液相色谱法 氟喹诺酮类 化妆品 

分 类 号：O657.72[理学—分析化学] R978.19[理学—化学]