水禽源禽1型副黏病毒强毒RT-PCR方法的建立  

Development of RT-PCR for detecting virulent strains of waterfowl-origin avian paramyxovirus type 1

作　　者：施少华[1,2] 程龙飞[1] 陈红梅[1] 傅光华[1] 杨维星[3] 黄瑜[1] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福建福州350013 [2]福建省畜禽疫病防治工程技术研究中心,福建福州350013 [3]江西农业大学,江西南昌330045

出　　处：《福建农业学报》2009年第1期11-13,共3页Fujian Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(30671564);国家"973"重大基础研究专项子课题(2005CB523001);福建省科技重大专项子课题(2006NZ0003-2)

摘　　要：参照GenBank上登录的水禽源禽1型副黏病毒强毒株F基因序列设计引物,建立检测水禽源禽1型副黏病毒强毒株的RT-PCR方法。该方法能从水禽源禽1型副黏病毒强毒株扩增出1条400 bp的特异片段,从水禽源禽1型副黏病毒弱毒、鸭瘟病毒、鸭1型肝炎病毒、减蛋综合征病毒、传染性法氏囊病病毒、H9亚型禽流感病毒和禽多杀性巴氏杆菌等均不能扩增出目的片段。敏感性试验显示该RT-PCR方法最低可检测出10 pg的病毒核酸。因此,该RT-PCR方法可用于水禽源禽1型副黏病毒强毒的临床诊断。An RT-PCR method was developed to detect virulent strains of waterfowl-origin avian paramyxovirus type 1 （vwAPMV-1）. The primers were designed according to the sequences of the fusion protein gene of vwAPMV-1s available at the GenBank. The result showed that the 400 bp of specific fragments were amplified in vwAPMV-1. However, negative results were obtained from Pasteurella multocide, EDS, DEV, IBDV, H9N2 influenza virus, DHV-1 and low virulent strains of waterfowl-origin avian paramyxovirus type 1. And, the 10pg of vwAPMV-1 RNA was amplified by the sensitivity test for the RT-PCR. The results indicated that the RT-PCR could be used for vwAPMV-1 detection.

关 键 词：水禽源禽1型副黏病毒 强毒株 RT—PCR 

分 类 号：S855.3[农业科学—临床兽医学]