姜黄素对巨噬细胞源性荷脂细胞胆固醇水平及caveolin-1表达的影响  

The Effect of Curcumin on the Cholesterol Metabolism and the Expression of Caveolin-1 in Macrophage-derived Cholesterol-load Cell

作　　者：李熠[1] 匡双玉[2] 王北冰[1] 尹凯[1] 沈元琼[1] 桂庆军[1] 

机构地区：[1]南华大学医学院诊断学教研室,湖南衡阳421001 [2]南华大学第二附属医院药剂科

出　　处：《南华大学学报（医学版）》2009年第1期18-20,共3页Journal of Nanhua University(Medical Edition)

基　　金：湖南省科技厅课题(编号:2007SK30092);湖南省卫生厅课题(编号:B2007092)

摘　　要：目的观察姜黄素对巨噬细胞源性荷脂细胞胆固醇水平的影响,并初步探讨caveolin-1蛋白表达在其中的作用。方法实验分组分为正常巨噬细胞组、巨噬细胞源性荷脂细胞组和姜黄素处理组。3组均采用油红O染色检测细胞荷脂情况,高效液相色谱分析法检测细胞内胆固醇含量,Western印迹法检测caveolin-1蛋白的表达。结果姜黄素作用于巨噬细胞源性荷脂细胞,细胞内总胆固醇、游离胆固醇和胆固醇酯含量均下降;caveolin-1蛋白表达上调。结论姜黄素引起巨噬细胞源性荷脂细胞胆固醇含量下降的同时,caveolin-1蛋白表达上调。Objective To observe the effect of curcumin on the cholesterol metabolism in macrophage - derived cholesterol - load cell, and to investigate the effect of the expression of caveolin - 1 in this process. Methods Three groups are designed in the experimmt as follows ： the normal macrophage group, the macrophage - derived cholesterol - load cells group and the macrophage- derived cholesterol -load cells group effected by curcumin. Rathonum red stain and high performanee liquid phase analytic process are used to assess and detect the level of cholesterol. The expression of caveolin - 1 is detected by Western Blot. Results With curcumin effects,the level of total,free and esterified cholesterol decrease in macrophage - derived cholesterol - load cell, while the expression of caveolin - t is up - regulated. Conclusion Curcurain can degrade the level of total, free and esterified cholesterol in macrophage - derived cholesterol - toad cell and it is contributed to the up - regulation of the expression of caveolin - 1 at the same time.

关 键 词：姜黄素 巨噬细胞 荷脂细胞 胆固醇 CAVEOLIN-1 

分 类 号：R541.4[医药卫生—心血管疾病]