热炎宁颗粒质量标准研究  

Study on the Quality Standard of Reyanning Granule

作　　者：陈冬利 龙洪 

机构地区：[1]郴州市妇幼保健院,湖南郴州423000

出　　处：《中医药导报》2009年第2期72-74,87,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:制定热炎宁颗粒的质量标准。方法:采用薄层色谱法对方中蒲公英、虎杖、半枝莲进行定性鉴别,采用高效液相色谱法对大黄素、野黄芩苷进行含量测定。结果:薄层鉴别均斑点清晰,阴性对照无干扰。含量测定中大黄素对照品进样量在0.0432-0.2160μg范围内,与峰面积线性关系良好(r=0.9983);其平均回收率为98.83%,RSD%为1.29%。野黄芩苷对照品进样量在0.04188-0.8376μg范围内,与峰面积线性关系良好(r=0.9998);其平均回收率为97.85%,RSD%为1.71%。结论:所建立方法操作简单,专属性和重现性良好,可作为该制剂的质量控制方法。Objective： To establish the quality standard of Reyanning granule. Methods： The TLC method was used to identify qualitation for Dandelion, Giant Knotweed Rhizome, Barbated Skullcup Herb; HPLC method was used to determine the contents of emodin and scutellarin. Results： the spot was clear in TLC identification without interference of negative control. The sample size of emodin had a good linear relation with peak area（r=0.9983）, when ranged from 0.0432-0.2160 μg, the average recovery was 98.83%, RSD%=1.29%; The sample size of scutellarin had a good linear relation with peak area（r=0.9998）, when ranged from 0.04188-0.8376 μg, the average recovery was 97.85%, RSD%=1.71%. Conclusion： The method is simple, with good specificity and reproducibility, can be used as the quality control for this preparation.

关 键 词：热炎宁颗粒 蒲公英 虎杖 半枝莲 大黄素 野黄芩苷 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]