正交设计法优选复方脑得生中总黄酮的提取工艺  

Extraction of total flavonoids from Naodesheng by orthogonal design

作　　者：王光忠[1] 肖锦[1] 李桥[1] 宋恬[1] 张明[1] 樊文娟[1] 刘焱文[1] 

机构地区：[1]"中药资源与中药复方"省部共建教育部重点实验室湖北中医学院,湖北武汉430065

出　　处：《中国当代医药》2009年第5期85-87,共3页China Modern Medicine

基　　金：武汉市科技局资助项目(No.20056002050-01)

摘　　要：目的:优选复方脑得生中总黄酮的提取工艺条件。方法:以总黄酮、葛根素及羟基红花黄色素A提取量为考察指标,用比色法测定总黄酮含量、高效液相色谱法测定葛根素及羟基红花黄色素A含量,以乙醇用量、乙醇浓度、提取时间、提取次数为影响因素,选用L9(34)表进行正交实验。结果:优选提取工艺为,70%乙醇,提取3次,提取时间(用量)为第一次2 h(10倍)、第二次1 h(8倍)、第三次1 h(6倍)。结论:优选工艺提取的黄酮类成分含量高,工艺简单,适宜工业化生产。Objective：To confirm the optimum extraction of the total flavonoids s from Naodesheng. Methods：The orthogonal test was used to optimize the extracting condition.Total flavonoids determined by colorimetr and puerarin and hydroxysafflor yellow A determined by HPLC were taken as the evaluating markers.The effects of different extraction methods on the extraction efficiency of the flavonoids were investigated. Results：The optimum extraction conditions were refluxed with 10,8,6 times amount of 70% alcohol and for 3 times（2 h,1 h,1 h）. Conclusion：The contents of the flavonoids obtained were high by the optimum extraction conditions .The technique is simple and adapts for production.

关 键 词：复方脑得生 总黄酮 提取工艺 正交实验 

分 类 号：R917[医药卫生—药物分析学]