雷公藤对糖尿病大鼠肾皮质GLUT-1 mRNA表达的影响及其意义  

Study on the effects of triptolide on the expression of glucose transporter-1 (GLUT-1) mRNA in renal cortex of the diabetic rats

作　　者：刘丽秋[1] 董晖[1] 周海燕[1] 王延萍[1] 

机构地区：[1]青岛大学医学院附属医院肾内科,山东青岛266003

出　　处：《中国老年学杂志》2009年第5期545-548,共4页Chinese Journal of Gerontology

基　　金：2007年度佳林豪基金项目

摘　　要：目的探讨雷公藤对糖尿病(DM)大鼠肾脏GLUT-1mRNA表达的影响及其意义。方法采用链脲佐菌素(STZ)腹腔注射法建立DM动物模型。将46只3月龄160～190g大鼠(雌雄各半)随机分为正常对照组,DM模型组,雷公藤组(6.7mg·kg-1.d-1)。第4、8、12周末各组处死4只大鼠并收集标本,记录体重、右肾重、检测血糖、血尿素氮、肌酐、24h尿蛋白定量。用RT-PCR方法检测肾皮质GLUT-1mRNA表达水平。肾组织行HE、PAS染色。结果①与正常对照组相比,DM模型组大鼠造模成功后血糖明显增高(P<0.01),但24h尿蛋白排泄量无明显增高(P>0.05);4w时体重、肾重指数、尿素氮、肌酐、24h尿蛋白排泄量、肾皮质GLUT-1mRNA表达均明显增加(P均<0.01),12w时增加更加显著。与DM模型组相比,雷公藤组8w时血糖、尿素氮、肌酐降低(P均<0.05),24h尿蛋白排泄量和肾皮质GLUT-1mRNA表达量均明显下降(P均<0.01);12w时体重增加(P<0.05),肾重指数才明显下降(P<0.01)。②肾组织HE、PAS染色病理学观察:DM模型组光镜下肾小球肥大,系膜细胞增生,系膜基质弥漫性或结节性增多,肾小球、肾小管基底膜增厚,而雷公藤组这些病理改变明显减轻。结论雷公藤能明显减少DM大鼠尿蛋白排泄量,抑制肾脏肥大、减轻肾脏的纤维化硬化程度。其机制可能是下调GLUT-1mRNA的表达量及功能活性,最终延缓DN的发展。Objective To investigate the effects of triptolide on the expression of glucose transporter （GLUT）-1 mRNA in the diabetic kidneys, and probe into its mechanism. Methods The rat diabetes model was established by intraperitoneal injection of STZ. Fortysix adult Wistar rats （half males and half females） ,3 months old, were divided randomly into normal control, diabetic model and triptolide treated groups （6.7 mg·kg^-1·d^-1）. Four rats of each group were sacrificed at the end of the 4th, 8th,12th week to collect samples for recordingbody weight, right kidney weight, blood sugar, serum creatinine, blood urea nitrogen ,24-hour urinary protein excretion. Renal GLUT-1 mRNA expression was measured with reverse transcriptase-polymerase chain reaction （RT-PCR）. Renal pathomorphology was observed by HE and PAS staining. Results ①Compared with normal control group, diabetic model group had the significantly increased blood sugar （P 〈0. 01） and had no significantly incresed 24 h urinary protein excretion （P〉0.05 ）at 0 w; had significantly decreased body weight （P〈0.01） and the significantly increased kidey weight index, serum creatinine, blood urea nitrogen,24 h urinary protein excretion, renal GLUT-1 mRNA expression （P〈0.01） at the end of 4th week; so did at the end of 12th week. Compared with model group, triptolide group had significantly decreased blood sugar, serum creatinine and blood urea nitrogen （P 〈0. 05 ）, 24 h urinary protein excretion and renal GLUT-1 mRNA expressions （P 〈 0. 01 ） at the end of 8th week ;and had significantly increased body weight（ P 〈 0. 05 ）and kidey weight index （P 〈 0.01 ） at the end of 12th week. ②HE and PAS dyeing showed that glomcrulus hypertrophia, mesangial cell proliferation, mesangial matrix diffused and nodular multiplication, thicking of the. glomerular and tubular basement membrane of rats inmodel group. In triptolide group these chages alleviated. Conclusions Triptolide can decrease the 24 h urinary protein excret

关 键 词：糖尿病 葡萄糖转运蛋白1 雷公藤 

分 类 号：R587.1[医药卫生—内分泌]