耐甲氧西林金黄葡萄球菌高变区基因分型及其与耐药性的关系  

The hyper-variable region genotypes of methicillin-resistant Staphylococcus aureus and their relationship with the resistance

作　　者：王敏[1] 李先平[1] 李文娟[1] 何斌[2] 

机构地区：[1]中南大学湘雅二医院检验科,长沙410011 [2]中南大学湘雅医学院医学检验系

出　　处：《中华微生物学和免疫学杂志》2009年第2期108-112,共5页Chinese Journal of Microbiology and Immunology

基　　金：湖南省自然科学基金资助项目（06JJ4051）

摘　　要：目的调查本地区耐甲氧西林金黄葡萄球菌（MRSA）的高变区（HVR）基因分型，分析HVR基因型与MRSA耐药谱的关系，并初步探讨其在分子流行病学分析中的作用。方法收集80株MRSA，采用PCR方法扩增MRSA的HVR，并根据扩增片段大小进行基因分型。同时统计各MRSA菌株对多种抗菌药物的药敏结果，并分析基因型与耐药性的关系。结果根据PCR产物片段大小，80株MRSA被分为A、B、C、D、E5种基因型，其中以D和E型多见，分别占61．25％和21．25％，A（3．75％）、B（5．00％）、C（8．75％）型少见。各基因型MRSA除对万古霉素敏感外，对头孢唑啉、庆大霉素、红霉素、阿奇霉素、克林霉素、环丙沙星、复方新诺明、阿米卡星的耐药率为61．5％～100％。结论MRSA的HVR基因分型是一种快速、简单、可靠的分型方法，适用于MRSA感染的流行病学调查，有利于抗菌药物的选用。Objective To investigate the hyper-variable region-polymerase chain reaction （HVRPCR） genotype of methicillin-resistant Staphylococcus aureus （MRSA） in local hospitals in Hunan province, and to compare it with the antibiograms, and to preliminarily discuss its role in molecular epidemiology of MRSA. Methods A total of 80 MRSA clinical isolates were collected from three affiliated hospitals of Central South University. Their DNA were extracted and amplified by PCR. The genotype was classified by the fragments of amplified products based on the size of HVR. The drug sensitivity test of MRSA was performed, and the correlation of genotypes and antibacterial resistance was analyzed. Results Eighty strains of MRSA were divided into 5 HVR genotypes which were named as A, B, C, D and E respectively according to the size of the PCR products. The most common types were D （61.25%） and E （21.25%）, followed by A （3.75%）, B （5.00%） and C （8.75%）. Most strains of genotypes were multi-drug resistant but all strains were sensitive to vancomycin. Conclusion These results suggest that HVR-PCR genotype method is a rapid, convenient and effective method for epidemiological investigation of infections caused by MRSA, and it is also helpful for clinical selection of antibacterial agents in effective treatment of MRSA infection.

关 键 词：耐甲氧西林金黄葡萄球菌 基因高变区 分型 耐药性 

分 类 号：R446.5[医药卫生—诊断学]