中国HIV／AIDS患者T细胞及调节性T细胞CTLA4表达与疾病进展关系研究  

Relationship between CTLA-4 expression in T cells and regulatory T cells and disease progression of HIV infected Chinese

作　　者：张子宁[1] 王亚男[1] 张旻[1] 王哲[2] 姜拥军[1] 孙国权[1] 刘静[1] 金鑫[1] 韩晓旭[1] 尚红[1] 

机构地区：[1]中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室,沈阳110001 [2]河南省疾病预防控制中心

出　　处：《中华微生物学和免疫学杂志》2009年第2期170-174,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金（30600532）;国家科技重大专项课题资助项目（2008ZX10001-001）;国家重点基础研究发展计划973计划（2006CB504206）;辽宁省重点实验室项目（2008S242）

摘　　要：目的对中国HIV感染者T细胞及调节性T细胞CTLA-4表达与HIV疾病进展的相关性进行研究，探讨CTLA-4在HIV感染中的作用。方法选取58名HIV／AIDS患者（长期不进展组、无症状HIV组、AIDS组），应用流式细胞仪胞内染色技术检测T细胞及CD4^＋CD25^＋Foxp3^＋调节性T细胞内CTLA-4表达水平，分析其与CD4^＋T细胞、病毒载量、淋巴细胞活化凋亡水平的相关性。结果长期不进展组、无症状HIV组、AIDS组CD4^＋T细胞内CTLA-4表达水平依次增高（P〈0．05）；与CD4^＋T细胞显著负相关（P〈0．01），与CD8^＋T细胞活化（CD38表达）、凋亡水平（CD95表达）及CD4^＋T细胞凋亡水平显著相关（P〈0．05），与病毒载量无显著相关性。长期不进展组、无症状HIV组、AIDS组CD8^＋T细胞内CTLA-4表达水平差异无统计学意义；与CD4^＋T细胞、病毒载量、CD4^＋、CD8^＋T细胞活化及凋亡水平均无显著相关性。CD4^＋CD25^＋Foxp3^＋T细胞内CTLA-4表达水平长期不进展组明显低于无症状HIV组及AIDS组（P〈0．05）；与CD4^＋T细胞显著负相关（P〈0．05）；与CD4^＋、CD8^＋T淋巴细胞活化（HLA-DR表达）显著相关（P〈0．01）。结论中国HIV感染者CD4^＋T细胞及CD4^＋CD25^＋Foxp3^＋调节性T细胞内CTLA-4表达水平与疾病进展及免疫活化状态显著相关，参与HIV感染免疫平衡的调节。Objective To detect the alternation of the level of CTLA-4 expression in T cells and regulatory T cells, and to study the relationship between CTLA-4 expression in T cells and regulatory T cells and disease progression of HIV infected Chinese. Methods Fifty-eight untreated HIV-1 infected patients were enrolled and the level of CTLA-4 expression in T cells and regulatory T cells were detected by flow-cytometry. CD4^＋ T cell numbers, viral load, level of CD95, HLA-DR, CD38 expression on T cells were measured to study the relationship between the level of CTLA-4 expression and disease progression of HIV infected patients. Results We found that the level of CTLA-4 expression in CD4^＋ T cells continuously increased in long term nonprogressors （LTNP）, asymptomatic HIV infected patients （HIV） and AIDS patients （ P 〈 0.05 ）. The level of CTLA-4 expression in CD4^＋ T cells was significantly correlated with CD4^＋ T cell counts, the frequency of CD8^＋ CD38^＋ T cells, CD4^＋ CD95^＋ T cells and CD8^＋ CD95^＋ T cells （ P 〈 0.05 ）. There had no difference in the level of CTLA-4 expression in CD8^＋ T cells among all groups and neither did we find the relationship between the level of CTLA-4 expression on CD8^＋ T cells and the CD4 counts, viral load, activation or apoptosis of T cells. The level of CTLA-4 expression in regulatory T cells of LTNP group was significantly lower than that of HIV and AIDS group （ P 〈 0.05 ）. The level of CTLA-4 expression in regulatory T cells was significantly correlated with CD4 counts, the frequency of CD4^＋ HLA-DR^＋ T cells and CD8^＋ HLA-DR^＋ T cells （P 〈 0.05）. Conclusion The level of CTLA-4 expression in CD4^＋ T cells and regulatory T cells is correlated with disease progression and the level of the activation of immune system of HIV infected Chinese and may play a role in the immune balance in HIV infection.

关 键 词：HIV-1 调节性T细胞 CTLA-4 活化 凋亡 

分 类 号：R512.91[医药卫生—内科学] R392.4[医药卫生—临床医学]