临床分离肺炎链球菌的毒力基因及血清型分析  

Prevalence of virulence genes in Streptococcus pneumoniae strains isolated from clinical patients

作　　者：董一山[1] 黄文祥[1] 罗涛[1] 章成[1] 吴利先[1] 

机构地区：[1]重庆医科大学附属第一医院重庆市传染病寄生虫病学重点实验室,400016

出　　处：《中华微生物学和免疫学杂志》2009年第2期177-180,共4页Chinese Journal of Microbiology and Immunology

基　　金：重庆市市卫生局资助课题（04-2-103）

摘　　要：目的了解肺炎链球菌的5种常见毒力基因（psaA、pspA、nanA、ply、lytA）在临床分离株中的分布情况。方法以2006-2008年从临床分离133株肺炎链球菌为研究对象，采用PCR法对5种常见的毒力基因进行检测。结果lytA、psaA、nard、pspA、ply这5种基因在133株菌中的检测阳性率分别为94．7％、85．0％、84．2％、60．2％、82．7％；5种毒力基因在87株常见血清型菌中的阳性率分别为100％、87．4％、89．7％、67．8％、86．2％。结论5种毒力基因在本地区肺炎链球菌中的阳性检测率均较高，在常见血清型菌株中的阳性检测率较其他血清型高。这5种基因是肺炎链球菌致病的主要毒力基因，可能作为新型肺炎蛋白疫苗的候选基因。Objective To investigate the prevalence of virulence genes（ply, pspA, nanA, lytA,psaA） among Streptococcus pneumoniae recently isolated from clinical patients. Methods The 133 strains were isolated from patients in three teaching hospitals in Chongqing from 2006 to 2008. Polymerase chain reaction was used to screen for virulence genes （ply, pspA, nanA, lytA, psaA）. Results The positive rate of lytA, psaA, ply, nanA and pspA in 133 clinical isolates were 94. 7%, 85.0%, 82.7% , 84. 2% and 60.2%, respectively. The positive rates of the lytA, psaA, ply, nanA and pspA genes in 87 common serotypes isolates was 100%, 87.4%, 86.2%, 89.7%, 67.8%, respectively. Conclusion The total positive rates of five virulence genes in the 133 clinical strains were high. The positive rates of five genes in the common serotypes isolates were higher than those in the no-common serotypes. These genes are important viru- lence genes of Streptococcus pneumoniae. They could be candidates for protein vaccine of Streptococcus pneumoniae.

关 键 词：毒力基因 血清型 肺炎链球菌 聚合酶链反应 

分 类 号：R378[医药卫生—病原生物学]