低氧训练对大鼠心肌组织血管内皮祖细胞标记AC133表达的影响  

Effects of Hypoxic Training on the Expression of AC133 Protein in Cardiac Muscle of Rats

作　　者：郑澜[1] 杨小波[1] 刘铭[1] 邹志兵[1] 凡婷[1] 

机构地区：[1]湖南师范大学体育学院,长沙410012

出　　处：《中国运动医学杂志》2009年第2期139-141,共3页Chinese Journal of Sports Medicine

基　　金：国家自然科学基金资助(No.30671011);湖南师范大学青年优秀人材培养计划(2007年)

摘　　要：目的:探讨低氧训练对大鼠心肌组织血管内皮祖细胞标记AC133表达的影响。方法:健康雄性SD大鼠16只,随机分为2组:对照组、高住高练组。高住高练组采用10周递增负荷跑台运动训练,每周训练6天,运动量由第1周的速度为15m/min、持续时间为25min递增至第10周速度为28m/min、持续时间为50min,每周二、四、六在相当于海拔1500m的低氧环境中训练,并且在低氧环境中居住,低氧程度由第1周相当于海拔1800m递增至第10周相当于海拔3600m。采用免疫组织化学方法检测CD34,定位心肌组织微血管;采用免疫组织化学及免疫电镜方法,检测微血管上AC133蛋白表达。结果:CD34可较好地标记心肌组织微血管;对照组大鼠心肌组织微血管内皮上有微弱的AC133蛋白表达,高住高练组大鼠心肌组织微血管内皮上有较多的AC133蛋白表达。结论:大鼠心肌内皮祖细胞归巢参与血管新生可能是低氧训练促进心肌血管新生的机制之一。Objective To study the effects of hypoxic training on the expression of AC133 protein in cardiac muscle of rats. Methods 16 male SD rats were randomly divided into two groups. 10 weeks progressive treadmill exercise program were designed with intensity starts from 15m/min for 25min at the first week to 28m/min for 50min in the last week, and hypoxia degree from 1800m at the first week to 3600m in the last week. CD34 immunohistochemical technology was applied to locate the site of microvessel. AC133 immunohistochemical technology and immune electron microscopy were used to detect the expression of AC133 protein in rats＇ cardiac muscle. Results CD34 could preferably display microvessel in cardiac muscle. More AC133 protein were detected on microvessel in hypoxic training group, compared with less AC133 detected in control group. Conclusion Endothelial progenitor cells home might be the mechanism of hypoxic training angiogenesis in cardiac muscle.

关 键 词：内皮祖细胞 AC133 心肌组织 低氧训练 

分 类 号：R87[医药卫生—运动医学]