J亚群禽白血病病毒蛋鸡分离株SD07LK1全基因组核苷酸序列的比较分析  

Sequence analysis for the complete proviral genome of Avian Leukosis Virus(Subgroup J)strain SD07LK1 isolated from layers

作　　者：郭桂杰[1] 孙淑红[1] 崔治中[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018

出　　处：《微生物学报》2009年第3期400-404,共5页Acta Microbiologica Sinica

基　　金：国家科技支撑计划(2006BAD06A08);山东省中青年科学家奖励基金(2008BS07004)~~

摘　　要：【目的】分析我国ALV-J蛋鸡分离株的来源和进一步演变趋势。【方法】以J亚群禽白血病病毒(ALV-J)蛋鸡分离株SD07LK1感染的鸡胚成纤维细胞(CEF)基因组DNA作为其前病毒基因组模板,根据已发表序列设计合成9对引物,经PCR扩增出9段连续的、相互部分重叠的DNA片段和闭合环形前病毒两末端LTR的连接区段,并分别连入T载体进行克隆、测序。【结果】用DNAstar软件对测序结果进行剪辑和拼接,首次完成了ALV-J蛋鸡分离株SD07LK1的前病毒全基因组核苷酸序列。【结论】将该序列与另外已完成的全基因组序列的比较表明,ALV-J的整个基因组gag和pol基因相对保守,各毒株间对应基因的同源性分别在95.0%以上,env基因的同源性仅为88.6%～94.0%。[Objective] To study the source and trend in evolution of ALV-J strains from layers in China. [Methods] The genomic DNA extracted from chicken embryo fibroblasts （CEF） infected by ALV-J strain SD07LK1 isolated from layers was used as template to amplify the ALV-J proviral DNA by PCR. Nine continuous and overlapping fragments were amplified with nine pairs of primers according to published sequences, then cloned into the T vector and sequenced. [Results] The complete sequence of the whole genome of ALV-J Chinese strain SD07LK1 was first established. [Conclusion] Comparisons of SD07LK1 sequence with that of the other Avian leukosis viruse strains, by using DNAstar software, demonstrated that the genes gag and pol of ALV-J were relatively conservative, the nucleotide identity of all the strains was over 95.0%. However, the gene env identity was only in the ranges between 88.6 and 94.0%.

关 键 词：J亚群禽白血病病毒 蛋鸡 全基因组核苷酸序列 

分 类 号：S852.65[农业科学—基础兽医学]