应用自动诱导表达体系提高原核表达效率  

Higher Prokaryotic Expression Efficiency Achieved by Auto-induction Expression System

作　　者：彭树英[1,2] 吕宁[1] 贾淑玲[1] 孙健红[1] 张涌[1] 

机构地区：[1]西北农林科技大学生物工程研究所,杨凌712100 [2]淮北煤炭师范学院生物系,淮北235000

出　　处：《农业生物技术学报》2009年第1期138-143,共6页Journal of Agricultural Biotechnology

基　　金：国家高技术研究与发展计划(863)项目(No.2004AA213072)资助

摘　　要：自动诱导表达体系是依据T7表达菌株对于培养基中的碳源和能源的利用机制建立起来的,用于生产各种异源蛋白更加高效、便捷和经济。为提高重组蛋白在原核细胞中的表达效率,构建了以S基因抗原位点区作为目的基因的原核表达重组质粒pET-S1,并将其转化入大肠杆菌(Escherichia coli)BL21(DE3)菌株。用IPTG诱导表达系统和自动诱导表达系统分别对目的蛋白进行表达。结果表明,目的蛋白最高表达量分别占菌体总蛋白的25.9%和45.1%,表明自动诱导表达系统可明显提高表达效率。To obtain the higher expression efficiency of producing recombinant protein in Escherichia coil, the prokaryotic expressing vector pET-S1, which contained whole antigenic sites of S gene from swine transmissible gastroenteritis coronavirus （TGEV）, was constructed and transformed into E.coli strain BL21 （DE3）. The target protein was expressed by the IPTG inducing expression system and auto-induction expression system, respectively. The highest amount of target protein accounted for 25.9% and 45.1% of total bacteria protein, respectively, indicating that the expression efficiency was significantly improved by auto-induction expression system.

关 键 词：原核表达 pET-28a IPTG 自动诱导 

分 类 号：S188[农业科学—农业基础科学]