一个与水稻条纹叶枯病抗性基因紧密连锁的分子标记的鉴定  

Identification of A Marker Closely Linked to Rice Stripe Disease Resistance Gene in a Japonica Rice Variety

作　　者：周彤[1] 王磊[1] 程兆榜[1] 陈洁[1] 范永坚[1] 周益军[1] 

机构地区：[1]江苏省农业科学院植物保护研究所,南京210014

出　　处：《农业生物技术学报》2009年第1期144-147,共4页Journal of Agricultural Biotechnology

基　　金：农业部行业专项(No.nyhyzx07-051);国家支撑计划(No.2006BAD02A16和No.2006BAD08A04);江苏省农业科技自主创新资金项目(No.cx[08]129);江苏省重大科技攻关(No.BE2005301);江苏省333工程人才和江苏省自然科学基金(No.BK2005105)资助

摘　　要：针对水稻条纹叶枯病粳型(Oryza sativa ssp.japonica)抗源镇稻88尚无可供育种利用的分子标记的现状,以镇稻88和感病品种武育粳3号配组构建F2作图群体。在采用单分蘖鉴定和苗期接种鉴定方法对F2群体和F2:3家系分别进行抗性遗传分析的基础上,通过标记分析表明,RAPD标记OPO11与镇稻88中抗性基因紧密连锁,表现为共分离。使用该标记对徐稻3号等3个镇稻88衍生抗性品种进行分析,结果表明,RAPD标记OPO11可应用于镇稻88与武育粳3号组合及其衍生组合的分子标记辅助选择。Markers related to the gene of rice stripe resistance in Oryza sativa ssp.japonica Zhendao88 were screened. The inheritance of rice stripe resistance in F2 lines and F2：3 lines from the cross Zhendao 88/Wuyujing No.3 were studied using filler inoculation and inoculation at the seeding stage, respectively. A marker OPO11, which was coseperated or completely linked to rice stripe disease resistance gene in Zhendao 88, was obtained by RAPD analysis. The amplification results of OPO11 primer in several resistance japonica varieties, such as Xudao No.3, originated from Zhendao 88, were the same as that of Zhendao 88. The OPO11 marker linked to the rice stripe resistance gene in Zhendao 88 could be used in marker-assisted selected program for rice stripe resistance breeding.

关 键 词：水稻条纹叶枯病 水稻品种 抗性 分子标记 

分 类 号：S188[农业科学—农业基础科学]