小麦抗叶锈病基因Lr2c的SSR标记  

SSR Markers Linked to Leaf Rust Resistance Gene Lr2c in Wheat

作　　者：张娜[1] 闫红飞[1] 张英春[1] 李爱霞[1] 陈玉婷[1] 张立荣[1] 杨文香[1] 刘大群[1] 

机构地区：[1]河北农业大学植物保护学院植物病理系河北省农作物病虫害生物防治工程技术研究中心,保定071001

出　　处：《农业生物技术学报》2009年第1期148-152,共5页Journal of Agricultural Biotechnology

基　　金：国家重大基础研究发展规划(973)前期课题(No.2005CCA01600);国家自然科学基金(No.30170602)资助

摘　　要：选取抗叶锈病基因位于2D染色体上的TcLr2c等7个小麦(Triticum aestivum)近等基因系、感病亲本Thatcher及215株TcLr2c与Thatcher杂交F2代为材料,研究抗叶锈病基因Lr2c SSR分子标记。从筛选的29对位于小麦2D染色体的SSR引物中获得4对能够揭示Lr2c多态性的分子标记,通过215株TcLr2c×Thatcher F2群体验证,结果表明Xgwm261和Xgwm296与Lr2c紧密连锁,其距目的基因的遗传距离分别为1.9和3.6 cM,可用于小麦抗叶锈病分子辅助育种。To identify the SSR marker linked to wheat leaf rust resistance gene Lr2c, SSR analysis was performed based on three kinds of materials, 7 wheat （Triticum aestivum） near iso-genic lines （NILs） with Lr genes located on chromosome 2D including two multiple alleles of Lr2c, the susceptible control Thatcher and a population of 215 F2 plants derived from TcLr2c × Thatcher. Twenty-nine SSR primer pairs on wheat 2D chromosome were screened and four of them could generate polymorphisms to the other near isogenic lines. The population of 215 F2 derived from TcLr2c × Thatcher was tested and verified. Result showed that Xgwm261 and Xgwm296were tightly linked to the gene Lr2c with a distance of 1.9 and 3.6 cM, respectively, indicating that Xgwm 261 and Xgwm 296 can be used in molecular assisted breeding against wheat leaf rust.

关 键 词：小麦抗叶锈病基因 Lr2c SSR 分子标记 

分 类 号：S188[农业科学—农业基础科学]