纳米TiO_2对黄瓜叶围细菌群落的影响  

Effect of Nano-TiO_2 on the Bacterial Community of the Cucumber Phyllosphere

作　　者：常立艳[1] 王琦[1] 梅汝鸿[1] 

机构地区：[1]中国农业大学植物病理学系农业部植物病理学重点开放实验室,北京100094

出　　处：《农业生物技术学报》2009年第1期159-163,共5页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金项目(No.30640044和No.30871665);北京市自然科学基金(No.6073028)资助

摘　　要：利用可培养和变性梯度凝胶电泳(DGGE)方法,对经不同浓度TiO2处理黄瓜(Cucumis sativus)叶围细菌群落结构的变化进行了分析。研究发现,TiO2处理后黄瓜叶围可培养细菌数量较对照显著降低。随着TiO2浓度从0.02 mg/mL提高至20mg/mL,黄瓜叶围可培养细菌数量从1.8×107 cfu/g降低至3.1×106 cfu/g。DGGE指纹图谱分析表明,当TiO2的喷雾浓度超过0.02 mg/mL时,黄瓜叶围细菌的多样性显著降低。对DGGE条带克隆测序结果显示,在黄瓜叶围至少存在7种不同属的细菌,其中只有一种叶围细菌不受TiO2喷雾浓度影响。The impact ofnanometer titanium dioxide（nano-TiO2） to the phyllosphere bacterial community were assessed using culturable-depend and PCR-DGGE methods. Results showed that quantities of the culturable phyllosphere bacteria were reduced from 1.8×10^7 to 3.1×10^6 cfu/g with the increasing concentrations of the nano-TiO2 from 0.002 to 20 mg/mL. The diversity of the phyllo- sphere bacteria was also analyzed by PCR-DGGE. When the concentrations of the nano-TiO2 were higher than 0.02 mg/mL, the DGGE bands was significantly lower than that of the control. Sequencing results of the bands from the DGGE gel showed that there were at least 7 bacterial genera on the cucumber phyllosphere. But only one phyllosphere uncultured bacterium was not influenced by the concentration of the nano-TiO2.

关 键 词：纳米颗粒 叶围细菌 DGGE 16S RDNA 序列测定 

分 类 号：S188[农业科学—农业基础科学]