聚乙烯醇涂层对电极植入大鼠脑组织后神经胶质酸性蛋白表达的影响  

Effects of PVA Coating on the GFAP Expression in Adult Rat Brain Tissue after Implantation of Electrodes

作　　者：叶明[1] 李涛[1] 段晏文[2] 曹余良[2] 鲁艺[2] 李承晏[1] 

机构地区：[1]武汉大学人民医院神经内科,湖北武汉430060 [2]武汉大学化学与分子科学学院,湖北武汉430072

出　　处：《武汉大学学报（医学版）》2009年第2期141-143,155,I0003,共5页Medical Journal of Wuhan University

基　　金：国家863项目(编号:2006AA02Z4E6);国家自然科学基金(编号:30570516)

摘　　要：目的:探讨聚乙烯醇(PVA)涂层对电极植入脑皮层后神经胶质酸性蛋白(GFAP)表达的影响。方法:22只Wistar大鼠采用左右脑自身对照,分别植入纯硅胶电极(对照)和有PVA共聚物膜涂层的硅胶电极。植入后4周、8周进行病理检查,并用免疫组化方法观察脑组织GFAP的表达。结果:4周时,50-100μm及100μm外PVA涂层电极GFAP表达较对照电极少(P<0.05);8周时,PVA涂层电极在电极组织界面0-50μm、50-100μm及100μm外GFAP表达均较对照电极减少(P<0.05)。两组电极各自8周50-100μm及100μm外的GFAP表达均较自身4周时少。结论:PVA涂层可减少电极组织界面GFAP的表达强度,改善植入电极的生物兼容性。Objective： To explore the effects of polyvinyl alcohol （PVA） coating on brain tissue response to implanted electrodes by studying the expression of glial fibrillary acidic protein （GFAP）. Methods： Twenty-two Wistar rats were implanted respectively silica gel electrodes （as controls） and PVA coating electrodes in left and right cerebral cortex respectively for self control. The tissue reactivity was observed by HE staining after 4 and 8 weeks, and the expression of GFAP was evaluated by immunohistochemistry. Results： After 4 weeks, there were significant differences in GFAP expression between the two groups at the brain area of 50-100 μm and of 〉 100 μm far from the implanting point （PVA〈controls, P〈0.05）. After 8 weeks, the expression of GFAP in the SG electrodes was significantly higher than that of the PVA coating group at the 0-50 μm, 50-100 μm, and 〉100 μm area（P〈0.05）. In addition, the expression of GFAP after 4 weeks was significantly higher than that after 8 weeks at 50-100 μm and 〉100 μ（P〈0.05） respectively in both groups. Conclusion： PVA coating can decrease the expression of GFAP and may improve the biocompatibility.

关 键 词：聚乙烯醇 电极 生物兼容性 神经胶质酸性蛋白 

分 类 号：R318.08[医药卫生—生物医学工程]