SREBP-1和SREBP-2在Ⅰ型糖尿病大鼠肾脏中的表达  

Expression of SREBP-1 and SREBP-2 in kidney of type 1 diabetic rats

作　　者：郝军[1] 王晨[1] 吴海江[1] 赵松[1] 段建召[1] 史永红[1] 段惠军[1] 

机构地区：[1]河北医科大学病理学教研室,河北石家庄050017

出　　处：《中国病理生理杂志》2009年第3期566-571,共6页Chinese Journal of Pathophysiology

基　　金：河北省科技厅基金资助项目(No.07276170)

摘　　要：目的:探讨糖尿病大鼠肾脏脂质沉积和固醇调节元件结合蛋白-1和-2(SREBP-1,SREBP-2)的表达。方法:以Wistar大鼠建立链脲佐菌素Ⅰ型糖尿病模型,测定肾组织甘油三酯和胆固醇含量,采用油红O染色检测脂质沉积的定位;用免疫组织化学和Western blotting检测SREBP-1、SREBP-2蛋白的表达;SREBP-1 mRNA测定采用原位杂交技术。结果:成功构建了Ⅰ型糖尿病大鼠模型,甘油三酯含量在1、2、4、8周均出现了明显升高,而肾脏胆固醇含量没有明显变化。油红O检测发现糖尿病大鼠肾脏近曲小管上皮细胞内出现明显脂滴,而肾小球内未见有脂滴染色。免疫组织化学检测发现,SREBP-1定位于糖尿病大鼠肾脏近曲小管上皮细胞胞浆,肾小球内未见有棕黄色颗粒。Western blotting进一步检测了SREBP-1蛋白的表达,结果发现糖尿病大鼠肾组织在4个时点均呈高表达,相似地,SREBP-1 mRNA的检测应用原位杂交技术,结果证实其定位于肾小管上皮细胞胞浆,而肾小球内未见着色。与正常对照组相比,糖尿病组大鼠肾脏SREBP-1 mRNA表达明显升高,差异显著。结论:Ⅰ型糖尿病大鼠肾小管上皮细胞SREBP-1 mRNA和蛋白表达升高可能参与了肾脏代谢异常。AIM ： To investigate the expression of sterol regulatory element binding protein - 1 （ SREBP - 1 ） and sterol regulatory element binding protein - 2 （ SREBP - 2） in the kidney of type 1 diabetic rats. METHODS ： The triglyceride （TG） and cholesterol content in the kidney of experimental rats were measured by the assay kits and oil red O staining. Furthermore, the expressions of SREBP - 1 and SREBP - 2 protein were detected by the methods of Western blotting and immunohistochemistry. The analysis of SREBP - 1 mRNA was performed by in situ hybridization. RFSULTS： Renal triglyceride content was markedly higher in diabetic rats than that in normal rats and the result of oil red O showed that lipid deposited in the renal tubular epithelium. Immunohistochemistry and Western blotting presented similar results that SREBP - 1 protein was up - regulated in renal tubular epithelium in diabetic rats. On the other hand, SREBP - 2 protein didn＇t show difference between diabetic rats and normal control rats. In situ hybridization confirmed the increasing of SREBP - 1 mRNA in renal tubular epithelium in diabetic rats. CONCLUSION： Above results suggest that altered SREBP - 1 may play an important role in the pathogenesis of renal lipid accumulation in type 1 diabetic rats.

关 键 词：糖尿病肾病 脂沉积 固醇调节元件结合蛋白质类 

分 类 号：R587.1[医药卫生—内分泌]