缺氧对大鼠血管平滑肌细胞表达巨噬细胞游走抑制因子的影响  

Effect of Hypoxia on the Expression of Macrophage Migration Inhibitory Factor(MIF)in Rat Vascular Smooth Muscle Cells

作　　者：胡晓琳[1] 潘平喜[2,3] 杨丽[1] 刘小菁[2,3] 付华[1] 

机构地区：[1]四川大学华西医院内科,成都610041 [2]四川大学华西医院心血管疾病研究室 [3]四川大学华西医院人类疾病生物治疗国家重点实验室

出　　处：《四川大学学报（医学版）》2009年第2期260-262,267,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30470437;30870596);成都市科技局基金(批准号成科计[2008]10号)资助

摘　　要：目的观察缺氧对大鼠血管平滑肌细胞(VSMCs)表达巨噬细胞游走抑制因子(MIF)的影响,探讨缺氧在动脉粥样硬化等血管增生性疾病中引起动脉壁损伤的可能机制。方法将大鼠VSMCs细胞株A7r5细胞分别在缺氧(37℃、5%CO2、1%O2)及常氧(37℃5、%CO2、20%O2)条件下培养不同的时间(2 h4、h1、2 h2、4 h、48h),在相应时间点收集细胞总RNA及培养上清液。用半定量RT-PCR及ELISA方法分别检测MIFmRNA的表达及VSMCs MIF的蛋白水平变化。结果与常氧培养的对照组相比,缺氧不同时间的大鼠VSMCs的MIFmRNA表达均显著增加(P<0.05),并且合成MIF蛋白的水平也明显增加(P<0.05)。结论缺氧能诱导VSMCsMIF的表达,MIF的表达增加可能是缺氧参与动脉粥样硬化等血管增生性疾病发生的机制之一。Objective To investigate whether hypoxia could induce the expression of MIF in vascular smooth muscle cells （VSMCs）. Methods Rat vascular smooth muscle cells （A7r5 from ATCC） were cultured under hypoxia condition （37 ℃, 5% CO2, 1% 02） and normoxia condition （37 ℃, 5% CO2, 20% 02） for 2, 4, 12, 24 or 48 hours respectively. MIF mRNA expression was detected by reverse transcriptase and polymerase chain reaction （RT-PCR）. MIF protein expression was determined by Enzyme-Linked Immunosorbent Assay （ELISA）. Results Expression of MIF mRNA was up-regulated as early as 2 hours in VSMCs after being exposed to hypoxia condition, which remained the elevated level up to 48 hours. Meanwhile, MIF protein level increased after hypoxia treatment for 2 hours, and maintained elevated status up to 48 hours. Conclusion Hypoxia could significantly induce MIF expression in VSMCs. MIF might be an important factor in the patho-physiological response of VSMCs to hypoxia.

关 键 词：缺氧 巨噬细胞游走抑制因子 血管平滑肌细胞 

分 类 号：R543[医药卫生—心血管疾病]