抗粘病毒1和2′5′-寡腺苷酸合成酶1基因与系统性红斑狼疮的相关性研究  

The Correlation of Myxovirus Resistance 1 and 2′5′-oligoadenylate Synthetase 1 with Clinical Features of Systemic Lupus Erythematosus

作　　者：李敏[1] 杨南萍[1] 黄向阳[1] 赵臻怡[1] 张杰[1] 刘潇潇[1] 蒲晓英[1] 董建玲[1] 叶扬[1] 

机构地区：[1]四川大学华西医院风湿免疫科,成都610041

出　　处：《四川大学学报（医学版）》2009年第2期268-271,共4页Journal of Sichuan University(Medical Sciences)

摘　　要：目的探讨系统性红斑狼疮(SLE)患者外周血白细胞中抗粘病毒1(MX1)基因和2′5′-寡腺苷酸合成酶(OAS)1基因的实时定量表达水平与SLE临床表现及病情活动度的相关性。方法收集50例SLE患者,20例非SLE其他自身免疫性疾病患者,25例健康对照人群的临床资料,取外周血抽提总RNA并逆转录成cDNA,运用SYBR green dye I实时定量聚合酶链反应(QT-PCR)在ABI PRISM 7000基因测序仪上检测患者和对照组的MX1和OAS1定量表达水平(以ΔCt值表示)的差异,并与各临床指标及病情活动度进行相关性分析。结果①SLE患者总体的MX1 mRNAΔCt值(3.55±1.39)高于非SLE患者组(2.31±0.52,P=0.000)和正常人(2.23±1.05,P=0.000)。②SLE患者组的OAS1 mRNAΔCt值(4.45±1.56)高于非SLE患者组(3.03±0.76,P=0.000)和正常人(2.75±0.64,P=0.000)。③SLE患者组的OAS1 mRNAΔCT值与SLEDAI积分之间有相关性(r=0.338,P=0.019),与血浆IgA水平有相关性(r=0.475,P=0.001)④SLE患者组的MX1 mRNAΔCT值与SLEDAI积分之间无相关性(r=0.064,P=0.661),与甘油三脂(TG)、胆固醇(TC)、低密度脂蛋白(LDL)2、4 h尿蛋白均有相关性(r=0.428,P=0.003;r=0.383,P=0.009;r=0.394,P=0.007;r=0.316,P=0.025)。⑤在有关节炎的SLE患者中,其MX1和OAS1表达水平升高更明显(3.04±1.42,P=0.004;3.89±1.49,P=0.006)。结论MX1与OAS1在SLE患者中均有表达上调现象,OAS1 mRNA实时表达定量水平对SLE患者的病情活动度判断有意义。二者作为Ⅰ型干扰素诱导表达的基因在SLE发病中均有各自作用。Objective To investigate the expression of myxovirus resistance 1 （MX1） and 2＇5＇-oligoadenylate synthetase 1（OAS1） genes in the peripheral blood leukocyte of patients with systemic lupus erythematosus （SLE）, and to evaluated the relations between these genes expression levels and disease activity. Methods In this study, there were 50 SLE petients, 20 non-SLE patients with rheumatic dieases, and 25 normal controls. The peripheral blood samples of these patients were collected, and the expression levels （indicated as ACt value） of MX1 and OAS1 were measured by Sybr green dye based real-time quantitative PCR method. Results （1） The ACt value of MX1 expression in SLE patients was 3.55±1.39, which was significantly higher than those of non-SLE patients（2.31± 0.52,P=0. 000） and normal controls （2.23 ± 1.05, P = 0. 000）. （2） The ACt value of OAS1 expression in SLE patients was 4.45 ± 1.56, which also was significantly higher than those of non-SLE patients（3.03 ± 0. 76, P= 0. 000） and normal controls（2.75±0.64,P=0. 000）. （3）The ACt value of OAS1 was correlated with the SLEDAI scores（r=0. 338,P=0. 019）and serum IgA level. （4） The ACt value of MX1 was not correlated with the SLEDAI scores（r= 0. 064, P = 0. 661）, but correlated with the serum levels of triglyceride （TG）, cholesterol （TC）, low density lipoprotein（LDL）, albuminuria of 24 hours （r = 0. 428, P = 0. 003 ; r= 0. 383, P = 0. 009; r = 0. 394, P = 0.007 ;r= 0. 316, P = 0. 025）;（5） The ΔCt values of MX1 and OAS1 in the SLE patients with arthritis were significantly higher than those in non-arthritis SLE patients （3.04 ± 1.42, P= 0. 004; 3.89 ± 1. 49, P = 0. 006）. Conclusion The expression levels of both MX1 and OAS1 in SLE patients are up-regulated, the expression levels of OAS1 genes are associated with SLE disease activity. As IFN-induced genes, MX1 and OAS1 play their respective role in SLE.

关 键 词：红斑狼疮 系统性 抗粘病毒1 2'-5’寡腺苷酸合成酶1 荧光定量聚合酶链反应 

分 类 号：R593.241[医药卫生—内科学]