平阳霉素白蛋白微球栓塞动脉和体内降解的动态观察  

Embolization and Degradation of Arteries in vivo by Pingyangmycin Albumin Microspheres

作　　者：王莉莉[1,2] 高庆红[1,2] 郑根建[3] 王晓毅[1,2] 周岚[3] 王昌美[2] 温玉明[2] 

机构地区：[1]四川大学口腔疾病研究国家重点实验室,成都610041 [2]四川大学华西口腔医院头颈肿瘤外科 [3]浙江省台州市中心医院口腔科

出　　处：《四川大学学报（医学版）》2009年第2期279-283,共5页Journal of Sichuan University(Medical Sciences)

基　　金：四川省科技攻关项目(03SG022-101);浙江省台州市科委基金(022219)资助

摘　　要：目的研究平阳霉素白蛋白微球(PYM-AMS)诱导小动脉栓塞的作用和体内降解的过程。方法选择健康成年日本大耳白兔24只,采用区组随机法,在每只兔双耳的中央动脉分别注射入PYM水剂、PYM油剂、PYM-AMS油剂各0.26 mL,PYM浓度为5 mg/mL。分别于注射后第2 d、7 d1、4 d、21 d处死动物,动态观察注射药物后的动静脉改变和PYM-AMS的形态变化。结果PYM水剂组,血管壁和血管内皮细胞无明显改变;PYM油剂组,约14 d时,出现血管充盈略下降,21 d有血管内膜及血管内皮细胞增生;PYM-AMS油剂组,7 d后血管出现缩窄,远心端有小血栓,血管逐渐机化,约21 d时,血管闭锁,血流中断。微球在注射后14 d,有吸收改变,逐渐有炎细胞浸润、组织侵入而降解。结论PYM-AMS通过栓塞和缓释PYM的作用,诱导小动脉闭锁,在体内2周后出现降解,未引起剧烈的炎性反应。PYM-AMS可作为动静脉血管畸形治疗或恶性肿瘤的局部化学治疗的选择性药物参考。Objective To investigate the embolization effect of Pingyangmycin-albumin microspheres（PYMAMS） on small arteries and its process of degradation in vivo. Methods Twenty four Japanese white rabbits were randomly divided into four groups, 6 in each group. PYM hydrochloride ＋ 0.9% NaC1, PYM ＋ soybean oil, and PYM-AMS ＋ soybean oil were injected into the central auricular arteries of the rabbits in the three experimental groups, respectively, for about 30 seconds （0. 26 mL/per ear, which contained PYM 5 mg/mL）. The vessel samples were taken and examined at 2, 7, 14, and 21 days. Results The PYM＋ 0. 9% NaCl group had no significant vessel changes. In the PYM＋ soybean oil group, some endothelial cells dropped off at the 7^th day after injection. At the 21^st day, mild proliferation of endothelial cells and walls of central auricular arteries were observed, especially on the intima. But the lumen was still obvious and the blood flow was not blocked. In the PYM-AMS group, the central auricular arteries were narrowed at the 7^th day after injection. At the 21^st day, the vessels had sclerostenosis, and the blood flow was blocked. At the 14^th day, significant proliferation of endothelial cells and walls of central auricular arteries were observed. The surface of PYM-AMS was absorbed. At the 21^st day, the walls of central auricular arteries and some small veins proliferated obviously, and the arteries were sclerostenosed. Many smooth muscle cells, fibroblasts, and myofibroblasts in the original blood vessel lumen appeared. There were thrombi besides the PYM-AMS. Conclusion PYM-AMS may become an option for the treatment of large venous or arteriovenous malformations and for the local chemotherapy of malignant tumors.

关 键 词：平阳霉素 白蛋白微球 栓塞 降解 

分 类 号：R96[医药卫生—药理学]