猪带绦虫六钩蚴超微结构的观察  

Observation on the Ultrastructure of Taenia solium Oncosphere

作　　者：孙晓林[1,2] 才学鹏[2] 景志忠[2] 米晓云[2,3] 王佩雅[2] 陈怀涛[1] 

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室/甘肃省动物寄生虫病重点实验室,兰州730046 [3]新疆畜牧科学院兽医研究所,乌鲁木齐830000

出　　处：《中国寄生虫学与寄生虫病杂志》2009年第1期35-38,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家高技术研究发展计划(863计划)项目(No.2006A-COA207)~~

摘　　要：目的观察猪带绦虫六钩蚴的超微结构。方法猪带绦虫病患者驱虫,收集成熟虫卵。次氯酸钠法破卵壳后,等渗细胞分离液(Percoll)收集六钩蚴,人工肠液激活。热琼脂离心法制备电镜样品,透射电镜观察。结果猪带绦虫成熟六钩蚴呈卵圆形,(14～17)μm×(10～13)μm,表面有不规则的突起或皱褶。六钩蚴小钩从外至内由颗粒带、纤维层和芯髓组成。成熟六钩蚴具成肌细胞、小钩生发细胞和皮层细胞等几种细胞类型。结论猪带绦虫六钩蚴的超微结构和缩小膜壳绦虫(Hymenolepis diminuta)六钩蚴的超微结构相似,但小钩的结构不同;猪带绦虫六钩蚴有形成上皮的双核细胞。Objective To observe the uhrastructure of Taenia solium oncospheres. Methods Patients infected with Taenia solium were de-wormed by decoction arecae and pumpkin seeds to get the mature proglottids and collect eggs. The eggs were treated with sodium hypochlorite to break the eggshells. Oncospheres were collected in Percoll （isoosmotic solution） , and activated with artificial intestinal juice. The specimens were prepared with hot agar centrifugation for ultra-thin sections and observed with transmission electron microscopy （TEM） . Results T. solium oncosphere was in oval shape with a size of （14-17） μm × （10-13） μm. There were some irregular ecphymata or plicae on its surface. The hooks were composed of outer pellet layer, the middle fibrous layer and the central core marrow. Myoblasts, hookforming cells and cerebral cortex ceils were observed in the mature oncospheres. Conclusion The ultrastructure of Taenia solium oncosphere is similar to that of Hymenolepis diminuta, with difference in hooks. There are binucleate cells which play a role in forming epithelium in the development of oncospheres.

关 键 词：猪带绦虫 六钩蚴 超微结构 透射电镜 

分 类 号：R383.321[医药卫生—医学寄生虫学]