日本血吸虫童虫cDNA文库免疫学筛选及阳性克隆的初步鉴定  

Immunoscreening and Identification of Schistosoma japonicum Juvenile cDNA Library

作　　者：段新伟[1] 傅颖慧[3] 卢艳[2] 黄成玉[1] 鞠川[2] 徐斌[2] 许学年[2] 冯正[2] 胡薇[2] 

机构地区：[1]华东理工大学生物工程学院,上海200237 [2]中国疾病预防控制中心寄生虫病预防控制所,世界卫生组织疟疾、血吸虫和丝虫病合作中心,卫生部寄生虫病原与媒介生物学重点实验室,上海200025 [3]上海交通大学医学院,上海200025

出　　处：《中国寄生虫学与寄生虫病杂志》2009年第1期80-82,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家高技术研究发展计划(863计划)项目(No.2007AA02Z153);热带医学研究中心/美国国立卫生研究院(No.5P50AI39461);国家重点基础研究发展计划(973计划)项目(No.2003CB716804)~~

摘　　要：用日本血吸虫感染14d的小鼠血清免疫筛选日本血吸虫童虫cDNA文库,将获得的7个阳性克隆进行核苷酸序列同源性分析,结果显示其中一个克隆所测序列与日本血吸虫HSP70有很高的同源性(分值score=650),另有2个克隆所测序列分别与已报道的日本血吸虫FABP(score=229)和含锌指结构的CDGSH型蛋白样蛋白(score=246)明显同源,其余4个未找到已知的同源序列,为新基因。4个新基因序列已被GenBank接受(登录号为EU121231、202646、202647和202648)。The cDNA library of Schistosoma japonicum （Sj） juveniles was immunoscreened with the anti-serum from day 14 post-infection mice. The inserts of the seven positive clones were sequenced and analyzed for their homology in GenBank database. Results showed that one was highly homologous to the SjHSP70 （score=650）, two were significantly homologous to the SjFABP（score=229） and Sj CDGSH-type Zn finger-containing protein-like protein（score= 246）, and the other four were not homologous to genes in GenBank and thus identified as Sj novel genes. The sequences of the novel genes were submitted to GenBank and the accession numbers were obtained （EU121231, 202646, 202647 and 202648）.

关 键 词：日本血吸虫 童虫 CDNA文库 免疫筛选 抗原基因 

分 类 号：R383.24[医药卫生—医学寄生虫学]