微小隐孢子虫子孢子与宿主黏附相关蛋白的筛选  

Screening for Relevant Proteins Involved in Adhesion of Cryptosporidium parvum Sporozoites to Host Cells

作　　者：郭安[1] 尹继刚[1] 向梅[2] 刘贤英[2] 张岩[1] 陈启军[1] 

机构地区：[1]吉林大学人兽共患病研究所人兽共患病教育部重点实验室,长春130062 [2]吉林大学第二临床医院妇产科,长春130041

出　　处：《中国寄生虫学与寄生虫病杂志》2009年第1期87-88,共2页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金(No.30400324)~~

摘　　要：利用人结肠癌细胞(Caco-2 cell)对微小隐孢子虫子孢子噬菌体展示文库进行筛选。对筛选得到的噬菌体进行克隆及测序分析,共获得5个特异的基因片段,其中1个为已知的编码微小隐孢子虫重要的侵入和黏附相关蛋白CP2,另外4个编码功能未知的蛋白。筛选得到的蛋白可能参与子孢子与宿主细胞的黏附过程。The Cryptosporidium parvum T7 phage display library was screened by using Caco-2 cells. Five specific gene fragments were identified by blasting sequences in GenBank, one of which encoding the CP2 protein was previously identified as a surface molecule of sporozoites and involved in parasite invasion. The others are hypothetic proteins with unknown functions. Bioinformatic analysis of these proteins indicated that they may be involved in the host-parasite interactions.

关 键 词：微小隐孢子虫 T7噬菌体展示 筛选 黏附 

分 类 号：R382.3[医药卫生—医学寄生虫学]