变性高效液相色谱检测食品中蜡样芽孢杆菌  

Detection of Bacillus cereus by denaturing high-performance liquid chromatography in food

作　　者：郑秋月[1,2] 傅俊范[2] 孙哲平[1] 裴轶君[1] 齐震玉[1] 曹际娟[1] 

机构地区：[1]辽宁出入境检验检疫局,辽宁大连116001 [2]沈阳农业大学,沈阳110161

出　　处：《中国卫生检验杂志》2009年第2期255-257,共3页Chinese Journal of Health Laboratory Technology

基　　金：国家"十一五"科技支撑计划课题(2006BAK02A13)

摘　　要：目的:应用PCR结合变性高效液相色谱技术对食品中污染的蜡样芽孢杆菌进行快速准确的检测。方法:利用所报道的蜡样芽孢杆菌gyrB基因引物,PCR扩增,DHPLC检测PCR扩增产物。同时进行方法特异性、灵敏度、精密度试验。结果:DHPLC检测蜡样芽孢杆菌出现特异性样品吸收峰,未检测到蜡样芽孢杆菌的近源种及其他细菌的阳性吸收峰,检测灵敏度可达到10 cfu/ml。结论:本研究所建立的PCR结合变性高效液相色谱检测蜡样芽孢杆菌的方法,特异性好,灵敏度高,快速简便。Objective： To use PCR and denaturing high - performance liquid chromatography to identify Bacillus cereus rapidly. Methods： Primers for specific PCR amplification were designed based on the published sequence of the gyrB gene of Bacillus cereus. Then its specificity, sensitivity and stability were examined. Results：Using this method to detect Bacillus cereus and other Bacteriaes of Pseudomonas, only Bacillus cereus can be detected, and as little as 10 cfu/ml of Bacillus cereus can be detected. Conclusion： A PCR-DHPLC assay amplification targeting at the gyrB gene of bacillus cereus is a rapid, specific, sensitive and relatively simple method for the identification of bacillus cereus.

关 键 词：蜡样芽孢杆菌 gyrB基因 PCR 变性高效液相色谱 

分 类 号：O657.72[理学—分析化学]