离子色谱分离-鲁米诺-亚铁氰化钾偶合化学发光测定蔬菜中NO_2^-  

Determination of NO_2^- in vegetable by luminol-potassium ferrocyanide chemiluminescence system after separation by ion chromatography

作　　者：冯媛媛[1] 杨迎春[1] 吉爱军[1] 印红玲[1] 叶芝祥[1] 

机构地区：[1]成都信息工程学院资源环境系,成都610225

出　　处：《中国卫生检验杂志》2009年第2期329-330,379,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立测定痕量亚硝酸根的新方法。方法:通过离子色谱分离NO2-,利用NO2-在酸性介质中氧化亚铁氰化钾为铁氰化钾与鲁米诺-铁氰化钾化学发光反应相偶合,间接测定NO2-。结果:亚硝酸根浓度在5.0×10-4~1.0×10-5g/L范围内与发光强度成良好的线性关系(r=0.9983),检出限(3σ)为5.2×10-6g/L,相对标准偏差为3.0%。样品加标回收率为92.3%~103.0%。结论:方法应用于蔬菜样品中亚硝酸根的测定,结果满意。Objective：To develop a simple and sensitive method for the determination of trace nitrite. Methods：Determination of NO2^- by the chemiluminescence of luminol and potassium ferrocyanide after separation by ion chromatography, which based on the oxidation of K4 [ Fe（CN） 6 ] to K3 [ Fe（CN） 6 ] by NO2^- in acidic medium. Results ：There was good relationship （r = 0. 9983 ） between the CL intensity and the concentration of nitrite in the range of 5 x 10 -4 - 1 x 10 -5 g/L with the relative standard deviation （RSD） 3.0% （n = 11, c = 5 × 10^-5 g/L）. And the limit of detection is 5. 2 × 10^-6 g/L. Recoveries of samples are between 92. 3% - 103. 0%. Conclusion：The method has been applied to the determination of nitrite in vegetables with satisfactory results.

关 键 词：鲁米诺 亚铁氰化钾 离子色谱 化学发光 NO2^- 

分 类 号：O657.3[理学—分析化学]