抗新城疫病毒HN蛋白单链抗体基因的克隆与序列分析  

Cloning and sequence analysis of gene of single chain antibody to haemaglutinin protein of Newcastle disease virus

作　　者：黄艳艳[1] 路希山[2] 胡北侠[1] 徐栋[3] 李士娟 程凯慧[2] 潘宗海[2] 张秀美[1] 

机构地区：[1]山东省农业科学院畜牧兽医研究所,山东济南250000 [2]青岛农业大学动物科技学院,山东青岛266109 [3]山东农业大学动物科技学院,山东泰安271018 [4]济南市农业开发办公室,山东济南250000

出　　处：《畜牧与兽医》2009年第2期13-17,共5页Animal Husbandry & Veterinary Medicine

基　　金：山东省科技攻关项目(022020103-02)

摘　　要：从分泌抗新城疫HN蛋白单克隆抗体的杂交瘤细胞中提取纯化mRNA,经RT-PCR扩增抗体轻、重链可变区基因,然后与L inker连接组装成单链抗体基因(scFv),其排列方式为VL-linker-VH。结果,成功地构建了scFv基因,序列分析表明,scFv基因长为744 bp,其中VH基因长为366 bp,VL基因长为333 bp。单链抗体基因的构建为抗新城疫HN蛋白基因工程抗体的制备奠定了基础。The mRNA from hybridoma cells which secrete monoclonal antibodies to haemaglutinin （HN） protein of Newcastle disease virus （NDV） was purified. The heavy-chain variable region （VH） and light-chain variable region （VL） genes encoding the monoclonal antibody were amplified by RT-PCR, and then linked with Linker in the way of VL-linker-VH, thus constructing the single chain antibody （scFv） gene. Sequence analysis demonstrated that scFv gene was 744 bp in length and the VH and VL genes was 366 bp and 333 bp, respectively. The constructed scFv gene could further be used to express the antibody.

关 键 词：新城疫病毒 HN蛋白 单链抗体 序列分析 

分 类 号：S852.4[农业科学—基础兽医学]