牛体外受精胚胎培养技术研究  

Technological investigation on the bovine oocyte in vitro culture

作　　者：桑国俊[1,2] 余四九[1] 贺奋义[1,2] 郭海龙[2] 邱忠玉[2] 豆有院[2] 王珂[2] 丁春贤[2] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]甘肃省畜牧兽医研究所,甘肃平凉744000

出　　处：《畜牧与兽医》2009年第3期11-14,共4页Animal Husbandry & Veterinary Medicine

基　　金：甘肃省农业生物技术项目《家畜胚胎移植关键技术研究与应用》

摘　　要：在COCs体外培养过程中添加共培养物质和采用不同体积的液滴进行培养,以探讨牛卵母细胞的体外培养效果。结果表明,卵丘细胞、卵丘细胞+bFF共培养系统对胚胎发育均有促进作用,其中以卵丘细胞+bFF共培养系统对桑椹胚和囊胚的发育最好,其囊胚率为10.63%,显著高于对照组的7.23%(P<0.05),高于卵丘细胞组的8.50%(P>0.05)。用培养皿微滴培养(50μL)和用4孔板大体积培养(500μL)的试验表明,大体积培养比微滴培养有优势,桑椹胚和囊胚率均较高,其中囊胚率为10.23%,显著高于培养皿微滴培养的9.07%(P<0.01)。In order to develop an efficient culture system of COCs in vitro, two methods which one was co-culture system with cumulus cell and/or bFF, and another was different volume of culture medium with 50 and/or 500μL were investigated. The results indicated that the hlastocyst rate of co-culture system with cumulus cell ＋ hFF were significantly higher than the cumulus cell and control groups （ P 〈 0. 05 ）. In addition, the hlastocyst rate of culture medium volume of 500 μL were significantly higher than that of 50 p.L （P 〈 0.01 ）. Together, the co-culture system with cumulus cell and/or bFF and culture medium volume of 500 μL could improve the embryo development in vitro culture.

关 键 词：牛 卵母细胞 卵丘细胞 卵泡液 体外培养 

分 类 号：S823[农业科学—畜牧学]