IBV安徽分离株膜蛋白基因的克隆及序列分析  

Cloning and sequencing of M genes of infectious bronchitis virus isolated from Anhui Province

作　　者：毛火云[1,2] 潘玲[1] 张小飞[2,3] 廖俊伟[1,3] 尹秀凤[3] 黄显明[1,3] 陈申秒[1] 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036 [2]南京天邦生物科技有限公司,江苏南京211102 [3]江苏省农业科学院,江苏南京210014

出　　处：《畜牧与兽医》2009年第3期22-25,共4页Animal Husbandry & Veterinary Medicine

摘　　要：利用设计的1对特异性引物，通过RT-PCR方法扩增出4株鸡传染性支气管炎病毒（my）安徽地方分离株膜蛋白M基因全长片段并进行了克隆测序。将各IBV安徽地方分离株与GenBank中注册的一些毒株M基因核苷酸序列及推导的氨基酸序列进行比较和系统进化关系分析，发现毒株间核苷酸序列同源性为88．5％～100％，其相应的氨基酸序列同源性为90．3％～100％；不同毒株间存着重组、缺失、插入及点突变等变异，从ATG至第140bp区段的核苷酸序列变异频率最高；4株分离毒株属于同一个进化群的2个不同进化亚群，与我国常用疫苗毒株H120、M41和W93不属同一个进化亚群。A pair of primers were designed and synthesized according to the published matrix （M） protein gene sequences of infectious bronchitis virus （IBV）. The full lengths of M genes of 4 strains of IBV isolated from Anbui were amplified by RT-PCR. The amplified prod- ucts were cloned and sequenced. The nueleotide sequences and the deduced amino acid sequences of M protein genes from the above isolates were compared with the published sequences of reference strains in GenBank, and the phylogenetic tree was constructed. The results showed that there were 88. 5% to 100% of nucleotide sequence homology and 90. 3% to 100% of deduced amino acid sequence homology among the strains. There were recombinations, deletions, insertions or mutations in the IBV isolates from Anhui. Furthermore, the fragment from start codon ATG to 140 bp showed the most variation; on the basis of the phylogenetic tree of M gene, it appeared that the IBV isolates in Anhui could be divided into two genetic subgroups, and were not in the same subgroup as the vaccine strains H120, M41 and W93 in china.

关 键 词：传染性支气管炎病毒 安徽分离株 膜蛋白基因 克隆 序列分析 

分 类 号：S852.6[农业科学—基础兽医学]