农杆菌介导的世锦B悬浮细胞团转基因体系的初步建立  

Preliminary Establishment of Agrobacterim-mediated Genetic Transformation system of Suspension Cells of a Rice Line "Shijin B"

作　　者：黎晶晶[1] 官杰[1] 陈莹[1] 李绍波[1] 余潮[1] 朱友林[1] 

机构地区：[1]南昌大学生命科学与食品工程学院,江西南昌330031

出　　处：《作物杂志》2009年第1期81-84,共4页Crops

基　　金：江西省科技计划攻关重点项目(20061B0203105)

摘　　要：本试验初步建立了农杆菌介导的世锦B悬浮细胞团转基因体系。利用世锦B成熟种子诱导愈伤组织并悬浮培养,得到生长旺盛的细胞团。将细胞团先在N6Ⅱ培养基上预培养1周,再用农杆菌侵染并在N6Ⅱ培养基上共培养,在含头孢霉素250mg/L、羧苄青霉素250mg/L、潮霉素50mg/L的N6I培养基上筛选,抗性愈伤接入MSIC分化培养基中,成苗后接入MSⅡ培养基中长成植株。抗性植株经PCR鉴定表明,外源基因已经整合到世锦B的基因组中。Agrobacterim-mediated genetic transformation system of a rice line ＂ShijinB＂ suspension cells was established. The callus was induced by the mature seeds of ShijinB, followed by suspension culture to obtain dynamic cells. The ceils were manipulated as follows ： pre-cuhured for a week, co-cultured in N6 Ⅱ medium, transferred to N6I medium including cefotaxime 250 mg/L, carbenicillin 250 mg/L and hygromycin 50 mg/L for screening, and transferred onto MSIC medium for differentiation. Plantlets obtained from resistant callus were transferred to MS Ⅱmedium. PCR amplification indicated that the exogenous gene was introduced into the rice genome.

关 键 词：水稻 农杆菌介导 遗传转化 悬浮细胞团 

分 类 号：S511[农业科学—作物学] X53[环境科学与工程—环境工程]