不同铜、锰水平对荷斯坦种公牛血清抗氧化指标的影响  

Effects of Different Levels of Copper and Manganese on Plasma Parameters of Holstein Bulls

作　　者：庄怀飞[1,2] 侯明海[2] 李彦芹[2] 王玲玲[2] 宋杰[2] 仲跻峰[2] 李文立[1] 

机构地区：[1]青岛农业大学动物科技学院,青岛266109 [2]山东省农科院奶牛研究中心,济南250100

出　　处：《中国农学通报》2009年第4期1-5,共5页Chinese Agricultural Science Bulletin

基　　金：山东省良种产业化项目"奶牛优秀公牛种质高效扩繁和克隆技术"(2007LZ015-04);农业部公益性行为(农业)科研专项"奶牛良种扩繁技术与产业化开发"(nyhyzx07-036-09);山东省农业重大应用技术创新"生态高效奶牛生产重大应用技术创新与集成应用"资助

摘　　要：旨在研究不同铜、锰水平对荷斯坦种公牛血清抗氧化指标的影响。试验选取40头荷斯坦种公牛,随机分为10组,对照组饲喂基础日粮,试验组采用3×3试验设计,分别饲喂不同铜、锰水平的日粮,铜的添加水平分别为4、8、12mg/kg(按日粮干物质计),锰的添加水平分别为50、125、200mg/kg,试验期8个月。结果表明:不同铜、锰水平对荷斯坦种公牛血清中谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)、锰超氧化物歧化酶(Mn-SOD)活性及血清一氧化氮(NO)含量差异极显著(P<0.01),对总抗氧化能力(T-AOC)、铜锌超氧化物歧化酶(CuZn-SOD)差异显著(P<0.05),对血清过氧化氢酶(CAT),铜兰蛋白(CP)活性及丙二醛(MDA)含量无显著差异(P>0.05)。综合分析不同铜、锰水平下试验牛抗氧化指标的变化,铜的适宜添加水平为4～8mg/kg日粮干物质,锰的适宜添加水平为50mg/kg日粮干物质。The experiment was conducted to study the effect of different levels of copper and manganese onp lasma antioxidation parameters of Holstein bulls.Forty Holstein bulls were randomly divided into tent reatments.The control group was fed with basic diet the others were fed with three levels of copper 4,8,1 2 mg/kg（on the basis of dry matter）and three levels of manganese 50,125,200 mg/kg（on the basis of drym atter）in the basal diet Experimental design was used by 3×3 trial design.The experiment lasted 8 months.T he results indicated that the activities of GSH-P X,SOD,Mn-SOD and the content of NO was the extremelys ignificant（P〈0.01）when basic diet was added different levels of copper and manganese,the significantd ifference of the activities of T-AOC and CuZn-SOD（P〈0.05）,and not significant difference of the activities ofC P,CAT and the content of MDA（P〉0.05）.It was concluded that basal diet supplementing with 4-8mg/kgc opper and 50 mg/kg manganese were the optimal supplementation in this experiment according to analysis ofa ll blood antioxidation parameters.

关 键 词：铜 锰 荷斯坦种公牛 抗氧化指标 

分 类 号：S823.99[农业科学—畜牧学]