家兔子宫内膜细胞炎症模型的建立  

Inflammatory Model of Endometrial Cells in Rabbits

作　　者：杜金梁[1] 徐丽娜[1] 马玉忠[1] 秦建华[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001

出　　处：《中国农学通报》2009年第4期18-20,共3页Chinese Agricultural Science Bulletin

基　　金：国家科技支撑计划"华北农区奶牛疾病综合技术的应用与示范"(2006BAD04A10-4)。

摘　　要：胶原酶消化法分离和培养家兔子宫内膜细胞,大肠杆菌细菌脂多糖(LPS)诱导子宫内膜细胞炎症,以培养细胞的形态学和传统炎症指标作为炎症发生和发展的判断标准。以不同浓度的LPS作用于细胞,收集不同时段培养上清液,ELISA法测定TNF-α、IL-1β的含量。结果表明,100ng/ml LPS是体外培养的子宫内膜细胞诱导炎症的最适浓度。炎症模型组培养上清液中TNF-α、IL-1β的含量比同期空白组高(P<0.01),表明炎症模型建立成功。The primary cultured endometrial cells in rabbits were isolated and purified by collagenased igestion method,cultured in vitro and induced inflammation by lipopolysaccharide（LPS）.The morphologya nd traditional inflammation factors of the cells were measured as the development criteria of inflammation.T he cells were treated with gradient concentrations of LPS,the culture supernatants were collected in differentp eriod,and the contents of TNF-α,IL-1βwere determined by ELISA.The results showed that 100 ng/mLL PS was the optimal concentration to induce the inflammation of cultured endometrial cells.The supernatantT NF-α,IL-1βin the inflammatory model group were higher than those in control at the same period（P〈0.01）,the inflammatory model of the endometrial cells was created successfully.

关 键 词：家兔 子宫内膜细胞 LPS 炎症模型 

分 类 号：S852[农业科学—基础兽医学]