MEF2a基因在肌肉组织中的表达研究  

Research on Expression of MEF2 Gene in Muscle Tissue

作　　者：高智晟[1,2] 杨龙[2] 张冬杰[2] 刘娣 刘玉芬[1] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院,哈尔滨150025 [2]黑龙江省农业科学院,哈尔滨150086

出　　处：《中国农学通报》2009年第4期21-23,共3页Chinese Agricultural Science Bulletin

基　　金：黑龙江省博士后基金资助项目"野猪;家猪及杂种猪的MEF2基因的克隆及表达规律的研究"(LBH-Z06231);黑龙江省杰出青年基金项目"野家杂交猪育种系与经济性状相关的功能基因的研究"(JC-05-19)。

摘　　要：分别取90日龄、180日龄和270日龄大白猪心肌、背肌和腿肌,提取总RNA,设计并合成猪MEF2a引物,以β肌动蛋白作为内参,利用Real-time PCR方法检测不同日龄大白猪肌肉组织中MEF2a基因mRNA的表达。结果表明,不同日龄大白猪的心肌和背肌中,MEF2a基因mRNA的表达水平无显著差异,在腿肌中有显著差异(P<0.05)。从90日龄到270日龄,MEF2a基因在心肌、背肌和腿肌的表达水平均差异显著(P<0.05),腿肌最高,其次是背肌,心肌最低。据此推测,MEF2a基因在猪成体后的肌肉组织中,主要是促进Ⅰ型肌纤维的生成,而不再是介导细胞分化。The expression levels of 90d,180d and 270d Landrace cardiac muscle,dorsal muscle and legm uscle were determined by Real-time PCR.Total RNA was extracted from muscle.The MEF2a PCR primersw as designed according to blast homologat the conserved regions.β-actin was used as loading controls.Ther esults showed that the cardiac muscle and dorsal muscle mRNA levels were not significantly varied amongd ifferent day,expect leg muscle（P〈0.05）.From 90d to 270 d,MEF2a expressions level were significant varieda mong cardiac muscle,dorsal muscle and leg muscle（P〈0.05）,and the highest is leg muscle,the second isd orsal muscle,the lowest is cardiac muscle.It was deduced that the main function of MEF2a is to promote Im yofibrillar formation and not to mediate cell differentiation during adult pig muscle.

关 键 词：猪 MEF2a基因 肌肉发生 REAL-TIME PCR 

分 类 号：Q953[生物学—动物学]