桃花粉管通道法转基因技术的初步研究  

A Preliminary Study on Transgenic Technology of Pollen-tubepathway in Peach(Prunus persical.Batsch)

作　　者：万春雁[1] 韩明玉[1] 赵彩平[1] 侯玉珏[1] 丛芳[1] 田玉命[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《中国农学通报》2009年第5期38-42,共5页Chinese Agricultural Science Bulletin

基　　金：农业部948项目"桃及桃加工技术引进和种质创新"(2006-G30);陕西省科技攻关项目"桃;油桃特色新品种引进与选育"(2008K01-11)资助

摘　　要：以曙光为试材,对桃花粉管通道法转基因技术进行探索性研究,结果表明:(1)活体人工授粉后,24 h花粉开始在柱头上萌发授粉后48 h已有大量的花粉管长至花柱上端;授粉后96 h大量的花粉管生长至花柱的中部;授粉后144 h大量的花粉管到达花柱基部。(2)确定外源DNA的注射时间为人工授粉后的144 h;花柱横切滴加法注射方式不适用于桃;当外源质粒DNA浓度为0.2 mg/L时,发育期内其坐果率都显著高于其它浓度水平。Experiment was conducted with Shuguang（Prunus persical.Batsch） for studying the applying of Pollen-tube path way in peach.Results showed： （1）24 h after pollination in vivo, pollens germinated on the stigma;After 48 h ,96 h,144 h plenty of pollen-tubes respectively reached to the upper,middle,bottom of style. （2） The best time to inoculate was 144 h after pollination; The injection method of adding the crosscut on the stigma was not suitable for peach;The rate of fruit setting was better than other concentration when was 0.2 mg/l.

关 键 词：桃 花粉管通道法 注射时间 注射方式 注射浓度 

分 类 号：S662.1[农业科学—果树学]