单细胞凝胶电泳技术检测蜘蛛血细胞DNA损伤研究  

Study on DNA Damages of Hemocytes in Spider with Single Cell Gel Electrophoresis

作　　者：李锐[1] 李生才[1] 杨怀卿[2] 刘佳[1] 罗原[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]山西农业大学现代教育科技学院,山西太谷030801

出　　处：《中国农学通报》2009年第5期48-52,共5页Chinese Agricultural Science Bulletin

基　　金：山西省青年基金"山西蜘蛛资源区系及保护利用研究"(2008021041);山西省科技攻关项目"农作物重大病虫害动态监测及综合治理新技术开发应用研究"(20080311023);"农作物重大病虫害监测预警技术研究"(2006031048)

摘　　要：以体外染毒法对星豹蛛雌、雄成蛛进行过氧化氢和甲醛染毒处理,采用单细胞凝胶电泳技术检测蜘蛛血细胞DNA损伤效应,用CASP软件分析彗星图像,并计算受损伤细胞率、彗星尾长和Olive尾矩。结果表明,不同浓度的过氧化氢能引起蜘蛛血细胞DNA断裂损伤,且损伤程度与浓度之间有明显的剂量-效应关系;不同浓度的甲醛能引起蜘蛛血细胞DNA损伤,其中在10μmol/L时引起DNA断裂,在25μmol/L、40μmol/L、55μmol/L时引起DNA交联,而在70μmol/L时引起血细胞凋亡。To study and evaluate genotoxicity of H2O2 and formaldehyde in the spider, adult of female and male spiders were treated with H2O2 at concentrations of 50, 75, 100, formaldehyde at 10, 25, 40, 55, 70μmol/L, respectively. DNA assay. CASP software was used to analyze the comet pictures. damage of hemocytes was Pardosa astrigera, the 125, 150 μmol/L and detected by the comet The results show that the percentage of comet, the percentage of tail length, and the tail moment of the H2O2 tested groups were significantly different from those of the control group （P〈0.05）. A statistically significant dose-dependent increase in DNA damage was observed. Formaldehyde induces DNA strand breaks at 10μmol/L, but crosslinks at 25, 40, 55μmol/L, and apoptoses at 70μmol/L in hemocytes cells of spiders.

关 键 词：过氧化氢 甲醛 血细胞 蜘蛛 DNA损伤 单细胞凝胶电泳 

分 类 号：S476.2[农业科学—农业昆虫与害虫防治]