人胰腺癌中前列腺干细胞抗原及紧密连接蛋白-4的表达  

Expression of Prostate Stem Cell Antigen and Claudin-4 in Human Pancreatic Carcinoma

作　　者：杨文彬[1] 蔡锋[2] 程传涛[1] 曹罡[1] 秦兆寅[1] 

机构地区：[1]西安交通大学医学院第二附属医院普外科,西安710004 [2]商丘市第一人民医院普外科,河南商丘476100

出　　处：《中国医学科学院学报》2008年第6期728-731,I0011,共5页Acta Academiae Medicinae Sinicae

摘　　要：目的检测前列腺干细胞抗原(PSCA)和紧密连接蛋白-4(Claudin-4)在胰腺癌中的表达情况,探讨PSCA和Claudin-4在胰腺癌发病中的作用。方法构建包含78例胰腺癌、12例慢性胰腺炎、10例正常胰腺组织的100点阵的组织芯片,免疫组织化学链霉菌抗生物素蛋白-过氧化物酶连接法检测该芯片中PSCA和Claudin-4的表达,分析其与胰腺癌临床病理因素的关系。结果78例胰腺癌患者中,PSCA和Claudin-4阳性表达率分别为79.5%和88.5%,与正常胰腺组织和慢性胰腺炎组织比较差异具有显著性(均P<0.01);PSCA和Claudin-4表达与患者年龄、性别及肿瘤分化程度、TNM分期无相关性。结论PSCA和Claudin-4阳性表达与胰腺癌相关,可能与胰腺癌的发生发展有着密切关系,但与胰腺癌的临床病理特型无关。Objective To investigate the expressions of prostate stem cell antigen （PSCA） and Claudin-4 in human pancreatic carcinoma and to discuss its role in the ontogenesis of pancreatic cancer. Methods Pancreatic carcinoma tissue microarray was constructed, containing 100 cores of 10 normal adult pancreas tissues, 12 chronic pancreatitis tissues, and 78 pancreatic carcinomas. The expressions of PSCA and Claudin- 4 were detected using immunohistoehemieal method and the relationship between PSCA and Claudin-4 and the pancreatic carcinoma was analyzed. Results The positive expression rates of PSCA and Claudin-4 protein in pancreatic carcinoma were 79.5% and 88.5% , respectively. PSCA and Claudin-4 staining were more intense in malignant cells than in chronic pancreatic tissues and normal adult pancreas tissues. No evidence was found for an association between expressions of PSCA and Claudin- 4 and other variables, including gender, age at surgery, and tumor grade. Conclusions The expressions of PSCA and Claudin- 4 are related to the pancreatic carcinomas. PSCA and Claudin-4 play a role in the development of pancreatic cancer, but PSCA and Claudin-4 are not correlated with the clinical pathology of tumor.

关 键 词：胰腺癌 前列腺干细胞抗原 紧密连接蛋白-4 组织芯片 免疫组织化学染色 

分 类 号：R657.5[医药卫生—外科学]