切应力对人骨髓间充质干细胞增殖的影响  

Shear stress stimulates mesenchymal stem cells proliferation

作　　者：孙阳[1] 易蔚[2] 魏旭峰[2] 易定华[2] 郭树忠[1] 

机构地区：[1]第四军医大学西京医院全军整形外科研究所,陕西西安710032 [2]第四军医大学西京医院心脏外科,陕西西安710032

出　　处：《中国美容医学》2009年第3期325-327,共3页Chinese Journal of Aesthetic Medicine

基　　金：国家自然科学基金资助项目(编号:30672086);国家863计划资助项目(编号:2006AA02A138)

摘　　要：目的:探讨切应力作用于人骨髓间充质干细胞(HMSCs)后,对细胞增殖的影响。方法:将HMSCs种植于平板流动腔中,待贴壁后对其施加1dyne/cm2的切应力作用6h,用MTT法测细胞增殖曲线,并用流式细胞仪测定细胞周期,计算增殖指数。结果:发现HMSCs在1dyne/cm2切应力作用6h后,增殖曲线抬高,G2+S期细胞明显增加。结论:HMSCs在切应力作用下,增殖能力增强,是组织工程血管种子细胞的理想选择。Objective To discover the effect of fluid shear stress on the proliferation of human mesenchymal stem cells （HMSCs）. Methods HMSCs were cultured on the bottom of the flow chamber for 1 day before exposure to fluid shear stress,and then exposed to 1 dyne/cm^2 shear stress for 6 h. We can acquire the proliferation curve by means of MTT and gain the cell cycle and cell proliferative index. Results Discovered that under the action of 1 dyne/cm^2 shear stress for 6 h, HMSCs improved their capability of proliferation. Conclusion HMSCs gain better ability of prolifeiation after exposure to shear stress, and they are promising seed cells in the field of tissue engineering blood vessels.

关 键 词：切应力 骨髓间充质干细胞 增殖 细胞周期 

分 类 号：Q813.1[生物学—生物工程]