DNT细胞分离鉴定及其在正常人外周血中含量的检测  

DNT cells isolated and identified in the peripheral blood and its content detection

作　　者：陈炯[1] 杜敏[1] 杨兵[1] 汤厚阔[1] 翟志敏[2] 李庆[2] 

机构地区：[1]安徽医科大学附属省立医院普通外科,合肥230001 [2]安徽医科大学附属省立医院中心实验室,合肥230001

出　　处：《国际外科学杂志》2009年第3期154-157,共4页International Journal of Surgery

基　　金：安徽省国际科技合作基金资助项目（No.6088015）;安徽高校省级自然科学研究重点项目（No.KJ2008A164）

摘　　要：目的建立人DNT细胞的免疫磁性分离（megnetic activated cell sorting，MACS）方法，并检测其在正常人外周血中含量。方法运用MACS法分离正常人外周血中DNT细胞，用台盼蓝染色流式细胞法检测选出细胞的活性和纯度，运用流式细胞法检测其在正常人外周血中含量。结果MACS分选法分选出的DNT细胞活性〉97％，纯度为82．77％；DNT细胞占TCRαβ＋T细胞的比例（6．25±2．61）％（n=45）。结论MACS法可简单迅速分选出高纯度DNT细胞，且不影响细胞活力。DNT细胞占TCRCD3^＋T细胞的比例为（6．25±2．61）％。Objective The immune magnetic separation （MACS） method of DNT cells was set up and their content was detected in peripheral blood of healthy man. Methods DNT cells were separated from the peripheral blood. Trypan blue staining and flow cytometry were used to detect their activity and purity, and flow cytometry was used to detect their content in normal human peripheral blood. Results DNT cell activity using MACS sorting was 〉 97%, the purity was 82.77% ; DNT cells accounted for （6.25 ± 2.61）% （ n = 45） in the TCRαβ ＋ T cells. Conclusion MACS can quickly sort out high purity DNT cells, and do not affect the vitality of cells. DNT cells accounted for （6.25± 2.61 ） % in the TCRCD3^＋ T cells.

关 键 词：DNT细胞 免疫磁性分离 外周血 

分 类 号：R446[医药卫生—诊断学]