促红细胞生成素对低氧培养的海马神经元凋亡的影响  

The Effect of Erythropoietin on Apoptosis of Hypoxia Cultured Hippocampal Neurons

作　　者：汪东良 黄树其[2] 常大川[2] 尹又[2] 

机构地区：[1]宁波市北仑区人民医院神经内科,浙江宁波315806 [2]上海长征医院神经内科,上海200003

出　　处：《中国神经免疫学和神经病学杂志》2009年第2期125-127,140,共4页Chinese Journal of Neuroimmunology and Neurology

摘　　要：目的探讨促红细胞生成素(EPO)对低氧条件下原代培养的海马神经元凋亡的影响及其可能机制。方法培养7 d的大鼠海马神经元随机分为常氧对照组、低氧对照组和重组人促红细胞生成素(rHuEPO)低氧处理组(简称rHuEPO处理组,又分100 IU/mL、150 IU/mL两亚组),以四唑盐(MTT)比色法测定培养12、24、36h的细胞存活率,以Western blot蛋白印迹法测定上述时间点B细胞白血病-淋巴瘤-2(Bcl-2)和Bcl-2相关X蛋白(Bax)表达。结果 EPO可明显增加低氧培养的海马神经元存活能力(P<0.01),但两剂量组之间差异无统计学意义;rHuEPO处理组神经元Bcl-2表达比同时间点低氧对照组明显增多(P<0.05),而Bax表达比低氧对照组明显减少(均P<0.01)。结论 EPO可明显增加低氧培养的海马神经元存活能力,其作用机制可能通过调控Bcl-2和Bax表达实现。Objective To explore the effect of erythropoietin（EPO） on primary cultured hippocampal neurons with hypoxia as well as its underlying mechanism. Methods On the 7th day after in vitro cultured, the hippocampal neurons from neonatal Wistar rats were randomized into normoxia and hypoxia control groups and rats in hypoxia group pretreated by 100 IU/mL and 150 IU/mL recombinant human erythropoietin（rHuEPO） respectively. The cell survival rate and the expression of B-cell non-Hodgkin lymphoma-2 （Bcl-2） and Bcl-2- associated X protein（Bax） were determined respectively by MTT assay and western blot. Results The survival rate of hypoxia cultured hippocampal neurons was enhanced significantly by EPO. However, there was no significant difference between two doses. The expression level of Bcl-2 was statistically higher in groups treated by EPO than the controls（P〈0.01）, in contrast, Bax was lower（P〈0.01）. Conclusions EPO could improve the survival rate of hippocampal neurons with hypoxia by regulating Bcl-2 and Bax.

关 键 词：促红细胞生成素 海马神经元 B细胞白血病淋巴瘤-2(Bcl-2) B细胞白血病-淋巴瘤-2相关X蛋 白(Bax) 

分 类 号：R338.1[医药卫生—人体生理学]