蓝黄青口服液对小鼠体外诱生细胞因子的影响  

The effect of induced secreting cytokine in vitro by LHQY

作　　者：伊鹏霏[1] 褚秀玲[1,2] 王新[1,3] 郭志廷[1] 韦旭斌[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]河北北方学院动物医学系,河北张家口075000 [3]青岛农业大学动物科技学院,山东青岛266109

出　　处：《中国兽医学报》2009年第3期323-326,共4页Chinese Journal of Veterinary Science

摘　　要：在对体外诱生小鼠脾淋巴细胞分泌细胞因子的影响试验中,用双抗体夹心ELISA法测定蓝黄青口服液(LHQY)对脾淋巴细胞分泌IL-2、IFN-γ及INF-α含量的影响,结果显示,LHQY在有无诱生剂ConA存在的条件均能极显著促进小鼠T淋巴细胞分泌IL-2和IFN-γ(P<0.01),加入诱生剂的组,分泌的量较大;在PHA-M的协同作用下,除药物浓度为1∶10外,均可极显著促进小鼠脾淋巴细胞分泌INF-α(P<0.01),但其本身对于INF-α的分泌主要起抑制作用。In the experiment to determine inducing leukomonocyte in vitro and the effect of secreting cytokine, determined the effect of LHQY on splenic lymphocyte secreting IL-2, IFN-γ and INF-α with double antibody ELISA. The results showed LHQY could significantly promote the secretion of IL-2 and IFN-γ with or without ConA by the T lymphocyte of mice（P〈0.01） ,and the level of IL-2 and IFN -γ was higher in the group adding ConA. With the PHA-M, except the drug concentration was 1 ： 10, LHQY could significantly promote the secretion of INF- α （P〈0.01） ,but it can inhibit the secretion of IFN-α by lymphocyte.

关 键 词：蓝黄青口服液 中药 细胞因子 

分 类 号：S859.7[农业科学—临床兽医学]