激素性股骨头坏死动物模型的建立及影像学评价  

Establish of animal models of steriod-induced osteonecrosis of the femoral head and evaluate by radiological methods

作　　者：张林波[1] 张桂珍[1] 杨海山[1] 柳林[1] 卜丽莎[1] 侯治富[1] 

机构地区：[1]吉林大学中日联谊医院,吉林长春130033

出　　处：《中国兽医学报》2009年第3期331-334,共4页Chinese Journal of Veterinary Science

基　　金：吉林省科委资助项目(20050401-5)

摘　　要：通过2种方法建立兔股骨头缺血坏死模型,并用影像学方法动态评价模型建立过程。30只日本大耳白兔随机分为3组,Ⅰ组为空白对照,Ⅱ组为单纯激素注射组(10 mg/kg),Ⅲ组为注射马血清(10 mg/kg)加激素(20 mg/kg);分别在第1、4、7周进行X线、CT、MRI、血管造影检查,取股骨头做组织病理学检查。结果显示,7周内3组X线、CT检查均为阴性;第7周C组血管造影显示病变部位血供减少,Ⅰ、Ⅱ组阴性;MRI检查第1周时Ⅲ组可见轻度骨缺血坏死改变,第4周时更明显,第7周时范围更广泛。Ⅱ组仅在第7周时见轻度骨缺血坏死改变。病理切片Ⅲ组在第1周时即有缺血坏死改变,第7周时范围和程度更明显;Ⅱ组在第7周时才出现轻度骨缺血坏死改变。结果表明,马血清加激素能更好地复制股骨头缺血坏死模型,优于单纯使用激素;MRI能及早发现股骨头缺血坏死改变,血管造影也能对股骨头缺血坏死范围和程度进行判定,为进一步活体研究提供指导。To establish of animal models of steriod-induced osteonecrosis of the femoral head by two ways and e valuate its changes by radiological methods. 30 healthy Japanese rabbits were randomly divided into three groups.Ⅰ group,6 animals, were injected the NS as the control group; Ⅱ group, 12 animals, were injected methylprednisolone （20 mg/kg） ; Ⅲ group, 12 animals,were injected horse serum（10 mL/kg） and methylprednisolone（20 mg/kg） step wise. At 1 week,4 weeks and 7 weeks after the last injection, 4 rabbits were examined with X-ray, CT, MRI, DSA and pathologic examination respectively. Negative was found on X-ray and CT in three groups. Vascular supply reduced in Ⅲ group at 7 weeks after injection and negative found in Ⅰ and Ⅱ group. Osteonecrosis of the femoral head were found at 1 week in group Ⅲ and 7 weeks in group Ⅱ ;There changes on MRI were consistent with the pathological changes. Ⅲ group show up most early and obviously osteonecrosis of the femoral head thanⅡgroup. It is possible for MRI to detect the early stages of bone osteonecrosis,the area and degree of bone osteonecrosis can be evaluated by DSA,and radiological examine may be prove guidelines for further research in live.

关 键 词：骨坏死 股骨头 动物模型 放射学 

分 类 号：S857.161[农业科学—临床兽医学]