高铜对雏鸡外周血T-淋巴细胞ANAE阳性率的影响  

Effect of high copper on the ANAE positive ratio of the peripheral blood T lymphocytes in chicken

作　　者：徐之勇[1,2] 彭西[1] 朱奎成[1] 邓俊良[1] 崔恒敏[1,3] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]河南科技学院动物科学学院,河南新乡453003 [3]四川农业大学都江堰分校,四川都江堰611830

出　　处：《中国兽医学报》2009年第3期350-353,359,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30471304);四川省教育厅;科技厅重点资助项目(2002A00203JY029-030-2)

摘　　要：选用1日龄艾维茵健康肉鸡420只,随机分为7组,分别喂以对照日粮(Cu 11 mg/kg)和高铜日粮(Cu 100mg/kg),高铜Ⅰ组,Cu 200 mg/kg,高铜Ⅱ组;Cu 300 mg/kg,高铜Ⅲ组;Cu 400 mg/kg,高铜Ⅳ组;Cu 500 mg/kg,高铜Ⅴ组;Cu 600 mg/kg,高铜Ⅵ组)6周,观察日粮铜添加水平对雏鸡外周血T-淋巴细胞ANAE阳性率的影响。与对照组比较,高铜Ⅰ、Ⅱ组雏鸡外周血T-淋巴细胞ANAE阳性率增加,但差异不显著;高铜Ⅲ、Ⅳ、Ⅴ、Ⅵ组雏鸡外周血T-淋巴细胞ANAE阳性率降低,且与日粮含铜量呈负相关,同时胸腺淋巴细胞减少,凋亡细胞比例增高。结果表明,与对照组比较,日粮铜含量100～200 mg/kg对雏鸡外周血中T-淋巴细胞ANAE阳性率有促进作用,但差异不显著;日粮铜含量300～600 mg/kg程度不同地降低了T-淋巴细胞ANAE阳性率,细胞免疫功能受损。The objective of this experiment was to evaluate effect of high copper on the ANAE positive ratio of the peripheral blood T-lymphocytes in chickens. 420 one-day-olg Avian chickens were randomly divided into sever groups,and fed on diets as follows： controls（Cu 11 mg/kg） and high copper （Cu 100 mg/kg,high copper proup Ⅰ; Cu 200 mg/kg,high copper group Ⅱ ;Cu 300 mg/kg,high copper group Ⅲ ;Cu 400 mg/kg,high copper group Ⅳ ;Cu 500 mg/kg,high copper group Ⅴ ;Cu 600 mg/kg,high copper group Ⅳ） for six weeks. The results showed that the ANAE positive ratio （%） of the peripheral blood T-lymphocytes in high copper groups Ⅰ and Ⅱhad no obvious difference comparied with control group. The ANAE positive ratio （%） of the peripheral blood T-lymphocytes in K high copper groups Ⅲ , Ⅳ, Ⅴ and Ⅳ wer decreased significantly compared with that of control group. It was concluded that dietary Cu more than 300 mg/kg could decrease the ANAE positive ratio （%） of the peripheral blood T- lymphocytes and impaire cellular immune function in chickens.

关 键 词：高铜 T-淋巴细胞 ANAE阳性率 胸腺病变 雏鸡 

分 类 号：S858.31[农业科学—临床兽医学] S816.72[农业科学—兽医学]