孔雀石绿分光光度法测加替沙星的含量  

Determination of gatifloxacin by spectrophotometric method with malachite green

作　　者：李维[1] 王超[2] 江虹[1] 

机构地区：[1]长江师范学院化学及环境科学系,重庆408100 [2]绵阳师范学院化学与化学工程系,四川绵阳621000

出　　处：《化学试剂》2009年第3期191-193,共3页Chemical Reagents

基　　金：重庆市教委科技基金资助项目(KJ051304);长江师范学院学生科研资助项目(07xky03)

摘　　要：研究了孔雀石绿与加替沙星的显色反应,建立了测定加替沙星的分光光度法。在pH 3.5～6.2的酸性条件下,孔雀石绿（MALA）与加替沙星（GATI）反应生成绿色配合物,最大吸收波长位于422 nm,表观摩尔吸光系数ε为3.68×10^4L·mol^-1·cm^-1,线性范围在0.2～11.3 mg/L,加替沙星在一定浓度范围内遵从朗伯比尔定律。方法用于实际样品的测定,回收率为98.2%～100.6%。The color reactions of malachite green with gatifloxacin were studied.A new spectrophotometric method for the determination of gatifloxacin has been developed.In an acidic condition with pH 3.5～6.2,malachite green（MALA） reacted with gatifloxacin（GATI） to form green complexes,the maximum absorption wavelength of which was identified at 422 nm and the apparent molar absorptivity（ε） was 3.68×104 L·mol^-1·cm^-1ith the linear ranges being at 0.2～11.3 mg/L.Gatifloxacin obeys the Lombard Beer′s law in a definite concentration range.This method was applied to determine the actual samples with a recovery of 98.2%～100.6%.

关 键 词：孔雀石绿 加替沙星 分光光度法 

分 类 号：O657.32[理学—分析化学]