猪细小病毒N株免疫期测定  

Determination on immune period of porcine parvovirus N strain

作　　者：梁保忠[1] 白安斌[1] 梁家幸[1] 赵武[1] 杜坚[1] 李斌[1] 黄安国[1] 姚瑞英[1] 蒋玉雯[1] 

机构地区：[1]广西兽医研究所,南宁530001

出　　处：《广西农业科学》2009年第2期207-209,共3页Guangxi Agricultural Sciences

基　　金：广西水产畜牧局科技项目(2002-54-35);广西自然科学基金项目(桂科自0728102)

摘　　要：用PPV-N株制备成弱毒疫苗,在配种前1个月接种PPV HI抗体阴性的后备母猪,接种2周后检测PPV HI抗体均达到1∶256以上。当怀孕到40d时用PPV强毒攻击,攻毒后40d剖杀的2头怀孕母猪其胎儿正常健活,胎儿心血未检测到PPV HI抗体,胎儿脏器未分离到病毒;自然分娩的2头母猪,其仔猪健活,未吃初乳仔猪PPV HI抗体阴性,仔猪脏器未分离到病毒;而强毒攻击的对照猪均出现不同程度的死胎,并从未吃初乳仔猪检测到PPV HI抗体和从死胎儿肺回收到病毒。按上述方法连续观察到第3胎(免疫后15个月),证明免疫母猪怀孕到第3胎仍能抵抗PPV强毒攻击,说明PPV-N株对母猪的免疫期达1年以上。The gilts with porcine parvovirus hemagglutination inhibition （PPV HI） antibody-negative were inoculated by attenuated vaccine of PPV-N strain in one month before mating, and PPV-HI antibody was up to 1：256 in two weeks after inoculating （PI）. The gilts were inoculated by virulent PPV on the 40th day after fetation, and two＇gravid sows were anatomized on the 40th day PI. Testing results showed their fetuses were alive, PPV HI antibody was not detected in the heart blood of fetuses, and PPV was not found in the viscera of fetus. Porkings propagated by two natural farrowing sows were alive. PPV HI antibody in porkings without suckling beastings was negative, and PPV was not found in the viscera of these porkings. But different dead fetuses were found in control sows inoculated by virulent PPV strain, the PPV HI antibody in these porkings without suckling beastings was positive, and PPV was found in the lung of these porkings. Test was performed continuously to the 3th fetation （15 months PI）, and the results showed that the inoculated sows in the 3th fetation could resist inoculation by virulent PPV, and the immunity period of PPV-N strain was more than one year.

关 键 词：猪细小病毒 N株 免疫期 

分 类 号：S858.282.6[农业科学—临床兽医学]